Dear Jerry,
Just a few comments on your questions.
We tried both SV and SE. SV showed an increasing Sw with decreasing
concentrations (Sw varied from 6.9, 7.0, 7.2, 7.4 to 7.522). IN addition, the
SV experiments showed a single boundary. However, the boundary was a little
broader, between 6
From: Kushol Gupta kushol.gu...@gmail.com
To: CCP4BB@JISCMAIL.AC.UK
Sent: Fri, May 27, 2011 9:51:04 AM
Subject: Re: [ccp4bb] larger molecular weight shown by analytic
ultracentrifugation
Hi Jerry –
By AUC, do you mean sedimentation velocity (SV)?
Both gel filtration
:57 -0700
From: cabrautc...@yahoo.com
Subject: Re: [ccp4bb] larger molecular weight shown by analytic
ultracentrifugation
To: CCP4BB@JISCMAIL.AC.UK
Dear Kushol Jerry,
I have to take exception to Kushol's contention about SV. As long as the
buffer and protein parameters are correct
Dear ALL;
I am sorry for this off-topic question about analytic ultracentrifugation
(AUC).
We recently solved one structure from crystals grown out of PEG4000 plus
buffer. Since the crystal was grown from PEG, we think the protein would
maintain its native oligomerization state as
