[ccp4bb] long needles

2009-08-25 Thread james09 pruza
Dear all, Sorry for the non-ccp4 question. Recently I have got some long needle of on 35 kDa protein with 65% MPD at 4 degree C at pH 3.6 (50 mM NaOAc buffer). The protein contains 50mM of salt. The florets are very thin. I need some suggestions regarding the optimization of the crystallization

Re: [ccp4bb] long needles

2009-08-25 Thread Enrico Stura
James and those that may be interested, 65% MPD at 4 degree C at pH 3.6 are conditions that stimulate the growth of salt crystals, but one cannot exclude that the florets are indeed protein. 1. If you are sure you are dealing with protein crystals try the co-precipitant approach: Carry out a