Dear all,
Sorry for the non-ccp4 question. Recently I have got some long needle of on
35 kDa protein with 65% MPD at 4 degree C at pH 3.6 (50 mM NaOAc buffer).
The protein contains 50mM of salt. The florets are very thin. I need some
suggestions regarding the optimization of the crystallization
James and those that may be interested,
65% MPD at 4 degree C at pH 3.6 are conditions that stimulate the growth
of salt crystals, but one cannot exclude that the florets are indeed
protein.
1. If you are sure you are dealing with protein crystals try the
co-precipitant
approach: Carry out a