[ccp4bb] Molecular replacement of a multidomain protein

Dear All, I am working with a Bifunctional protein of molecular weight ~60 kDa. I have a 3.3 angstrom native dataset. The matthews number show there are 6 molecules in the asymmetric unit. The structures of the individual domains are already known from prokaryotes. The sequence identity with the

Re: [ccp4bb] Molecular replacement

The answer to your question is called Phaser. Using Phaser, you can input multiple search models in one search routine to look for solutions. You should read the online manual and tutorials, if you are not already familiar with Phaser. You can also 'modify' or appropriately 'trim' your

[ccp4bb] Molecular replacement

Dear All, I wanted to solve one of my new structures by molecular replacement. I got three short search motifs from three different structures available in the PDB. My question is, how do I use all the search models to solve the structure. With by best regards, Sekar (\_/) (='.'=)

Re: [ccp4bb] Molecular Replacement of a Known Anomalous Substructure

Dear All, The authors of the following paper used molecular replacement of the substructure to locate the heavy atoms. The known 11-fold symmetry meant that the heavy atoms had to be in a circle - molecular replacement with circles of different radii did the trick. Antson et al. Nature,

Re: [ccp4bb] Molecular Replacement issues with a WD-40 7-bladed beta propeller

Hello Scott, hmmm - it is quite difficult to do a good analysis of your problem, remotely. You've tried the enantiomorphic space group I4(3), just to be sure? In principle, the molecular replacement solution given by Phaser sounds good, but this is no proof of whether it's correct. What

[ccp4bb] Molecular Replacement issues with a WD-40 7-bladed beta propeller

Hello, I'm an undergraduate and recently crystallized and obtained 2.9A diffraction data for a protein which is predicted to fold into a WD40 7-bladed beta-propeller structure (which has been crudely verified by cryo-EM by another lab). The space group appears to be I4(1) with unit cell

[ccp4bb] Molecular replacement and refinement trouble

Hi, Dear all, Firstly ,I'd like to thank all the people for their kind help, especially Miguel, Bernie ,Mark, Moody, Eleanor, Eaton , Peter and lots of others Without your help, I wouldn't make it this far. Secondly, I'd like to share some of the experiences. We had a hard time trying to do

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