crystallization experiments give you some more hints about the
solubility of the protein.
Good luck,
Jan
--- Katja Schleider *katjaschlei...@yahoo.de* schrieb am *Do,
19.11.2009:
*
*
Von: Katja Schleider katjaschlei...@yahoo.de
Betreff: [ccp4bb] off-topic: crystal optimization without
Hi everybody,
sorry for my off-topic question. I got small initial crystals in 200mM NaSulfat
and 20% PEG 3350.
There is no buffer in this condition. How can I optimize these crystals? Just
vary the PEG concentration? Or should I add a buffer; or vary the pH of the
buffer the proteinsolution
Forgot to hit the Reply all button (sorry :-( )
---BeginMessage---
Katja Schleider wrote:
Hi everybody,
sorry for my off-topic question. I got small initial crystals in 200mM
NaSulfat and 20% PEG 3350.
There is no buffer in this condition. How can I optimize these
crystals? Just vary the PEG
Hi Katja,
Sodium sulphate forms a solution of neutral pH in water.
200 mM of Na2SO4 will buffer the crystallization solution at pH 7, in
addition to acting as a salt. You could try varying the concentration of
PEG and Sod.sulphate now to see if the crystals get better. Sometimes using
. But this might change maybe if your
crystallization experiments give you some more hints about the solubility of
the protein.
Good luck,
Jan
--- Katja Schleider katjaschlei...@yahoo.de schrieb am Do, 19.11.2009:
Von: Katja Schleider katjaschlei...@yahoo.de
Betreff: [ccp4bb] off-topic: crystal optimization
