Re: [ccp4bb] pH-ing Jeffamine ED-2001

2008-05-19 Thread Clemens Grimm
this is also my experience. However, above pH 8, the buffering effect of the Jeffamines' amino groups starts to be significant and (rough) pre-adjusting of the pH might be necessary. Clemens Quoting Yogesh Gupta PhD [EMAIL PROTECTED]: Hi Amit, Changing the pH of Jeffamine is not so easy . I

Re: [ccp4bb] pH-ing Jeffamine ED-2001

2008-04-21 Thread Yogesh Gupta PhD
Hi Amit, Changing the pH of Jeffamine is not so easy . I would keep the pH of Jeffamine constant and rather change the pH of buffer. Anyway if Jeffamine concentration is too low as compare to the buffer you are using, it would not change the final pH much. This is what my experience with