Dear colleagues:
I was wondering if you could kindly share your thoughts and help me
understand the relationship between pKa and affinity of a protein for a
ligand. Are these two properties related? Specifically, does a lysine with
a pKa of 8.5 have a greater affinity for a negatively charged
A good starting place:
Biophys J. 1996 Oct;71(4):2049-55.
Evaluation of linked protonation effects in protein binding reactions using
isothermal titration calorimetry.
Baker BM, Murphy KP.
Abstract
A theoretical development in the evaluation of proton linkage in protein
binding reactions by
