Dear All:
I am sorry that I did not know the policy.
And thanks a lot for the kind reminder.
Jerry
CC: CCP4BB@JISCMAIL.AC.UK
From: [EMAIL PROTECTED]
Subject: Re: [ccp4bb] FW: [ccp4bb] salt sensitive complex
Date: Thu, 31 Jan 2008 09:37:01 +0100
To: [EMAIL PROTECTED]
Dear all
Dear All:
Firstly I would like to thank many folks here for giving me great ideas
several days ago.
The following are some updates for this question.
I did ITC experiments again using 25mMTris(pH8), 60mM NaCl(low salt
condition).
But things still turn out to be a
Hi Jerry,
to summarise your problem, using (close to) physiological buffer, SPR
and ITC give you different results, you get different results in
different salt strengths and to add to your misery, the proteins
precipitate at low salt concentrations when mixed to together.
Ok.
Given the above,
Dear All:
Recently I am pursuing the crystallziation of a complex formd by two
individual proteins and I met several interesting problems though they are
kind of off-topic.
Any suggestions for these problems will be highly appreciated.
BIAcore showed about
