[ccp4bb] sealing slides on VDX plates?

2011-11-18 Thread Dima Klenchin
Hello, I wonder what everyone is using for sealing hanging drop slides on VDX plates? For the most part, we paraffin oil but I am unhappy with it because it is too think and too frequently there is break in the oil and the drop dries too much. I find vacuum grease to be not terribly practical

Re: [ccp4bb] sealing slides on VDX plates?

2011-11-18 Thread Bosch, Juergen
Dow Corning Heavy Vacuum Grease, a 10 ml syringe and a shorter cut 200 µl tip attached to the syringe. Jürgen On Nov 18, 2011, at 12:55 PM, Dima Klenchin wrote: Hello, I wonder what everyone is using for sealing hanging drop slides on VDX plates? For the most part, we paraffin oil but I am

Re: [ccp4bb] sealing slides on VDX plates?

2011-11-18 Thread malhotra
Vaseline (pure, with no additives) from CVS/Walgreens in a 10 ml syringe and a pipette tip (wide bore) also works very well. On 11/18/11 12:58 PM, Bosch, Juergen wrote: Dow Corning Heavy Vacuum Grease, a 10 ml syringe and a shorter cut 200 µl tip attached to the syringe. Jürgen On Nov 18,

Re: [ccp4bb] sealing slides on VDX plates?

2011-11-18 Thread Prince, D Bryan
[mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of malho...@miami.edu Sent: Friday, November 18, 2011 1:26 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] sealing slides on VDX plates? Vaseline (pure, with no additives) from CVS/Walgreens in a 10 ml syringe and a pipette tip (wide bore) also works very

Re: [ccp4bb] sealing slides on VDX plates?

2011-11-18 Thread Christopher Browning
The is probably the cleanest and fastest way of greasing plates. Take a 15ml Falcon tube and unscrew the cap. You will notice that the cap is exactly the width as the well of the VDX plate. Using a fairly wide bored metal syringe needle, pierce 8 holes into the cap from the outside, along the

Re: [ccp4bb] sealing slides on VDX plates?

2011-11-18 Thread Patrick Loll
Haven't done this for a while, but what we used to do was mix Vaseline plus mineral oil (both purchased for cheap at the local drugstore), and then apply it using a 10 ml syringe with a pipet tip attached. We used the mixture of Vaseline + mineral oil because Vaseline alone is too viscous

Re: [ccp4bb] sealing slides on VDX plates?

2011-11-18 Thread Kay Perry
klenc...@facstaff.wisc.edu To: CCP4BB@JISCMAIL.AC.UK Sent: Friday, November 18, 2011 11:55:07 AM Subject: [ccp4bb] sealing slides on VDX plates? Hello, I wonder what everyone is using for sealing hanging drop slides on VDX plates? For the most part, we paraffin oil but I am unhappy