Re: [ccp4bb] seek your opion on this weird diffractio pattern

2020-12-08 Thread Kevin Jin
Hi Joseph, It is a very beautiful crystal with neat diffraction patterns and background, which may suggest a good cryoprotection. Here is my observation from your images. 1. From your image taken at 30 degrees (first page), I believe it is a protein crystal with a larger unit cell length on one

Re: [ccp4bb] seek your opion on this weird diffractio pattern

2020-12-08 Thread Robert Stroud
The first is a very interesting pattern! - It would be good to know more about exactly how the patterns were taken, camera length and dimensions to determine the spacings. - The first patterns look very strongly like Bessel functions of a helical diffraction pattern that is then sampled by a

Re: [ccp4bb] seek your opion on this weird diffractio pattern

2020-12-08 Thread Nave, Colin (DLSLtd,RAL,LSCI)
to recall the subject of PEG crystals coming up before. Colin -Original Message- From: CCP4 bulletin board On Behalf Of Joseph Ho Sent: 08 December 2020 15:47 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] seek your opion on this weird diffractio pattern Dear all: We recently worked on 8kDa

Re: [ccp4bb] seek your opion on this weird diffractio pattern

2020-12-08 Thread Jon Cooper
Hello, the first of those diffraction patterns is very beautiful. What was the concentration of zinc acetate? Also, did you keep the crystal? It might be worth prodding it with a needle and if holds together well or if you hear a loud crack it's probably salt. The second image shows a salt-like