Re: [ccp4bb] sodium thiocyanate as cryoprotectant

Hi All, Thank you very much for the suggestions! On Tue, Sep 27, 2016 at 4:37 PM, abhimanyu singh wrote: > Hi Alex, > > You may also think of gradually increasing PEG3350 concentration to around > 40% which has worked pretty well in some of my cases. > > Greetings, >

[ccp4bb] AW: [ccp4bb] sodium thiocyanate as cryoprotectant

bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Alex Lee Gesendet: Mittwoch, 28. September 2016 00:43 An: CCP4BB@JISCMAIL.AC.UK Betreff: [ccp4bb] sodium thiocyanate as cryoprotectant Hi CCP4bb members, I have a condition for my protein crystals at "0.2M sodium thiocyanate, 25% PE

Re: [ccp4bb] sodium thiocyanate as cryoprotectant

Hi Alex, You may also think of gradually increasing PEG3350 concentration to around 40% which has worked pretty well in some of my cases. Greetings, Abhi Abhimanyu Kumar Singh, Ph.D. School of Biosciences University of Kent Canterbury, UK. On 27 Sep 2016 23:43, "Alex Lee"

Re: [ccp4bb] sodium thiocyanate as cryoprotectant

Probably you can not use thiocyanate as cryoprotectant because its a chaotropic agent. Although its a very mild denaturant (destabilizer) but at high concentration, it can destroy your crystals. I have recently used 30 % sucrose for cryo protection of crystals growing under same condition and

[ccp4bb] sodium thiocyanate as cryoprotectant

Hi CCP4bb members, I have a condition for my protein crystals at "0.2M sodium thiocyanate, 25% PEG3350, pH6.9". I'd like to try cryoprotectant with just higher concentration of sodium thiocyanate, but I have no google hits of the range of concentration I should use. I wonder anyone in this