On Nov 13, 2010, at 11:04 PM, Jerry McCully wrote:
Dear ALL;
A few weeks ago, I posted my problem with MBP fusion protein.
Thank folks very much for the help. Here is my latest result.
1) Shortening the linker region would signifcantly reduce the cleavage of
target proteins
Dear ALL;
A few weeks ago, I posted my problem with MBP fusion protein.
Thank folks very much for the help. Here is my latest result.
1) Shortening the linker region would signifcantly reduce the cleavage of
target proteins from MBP;
2) Adding a C-terminal tag would minimize the
Hi,
I've seen soluble aggregates many times, with MBP and without. It's one of
the common outcomes of partially successful (sounds better than saying
'failed') expression attempts.
It is always possible to push further and try some fairly esoteric stuff
with E. coli if you're truly desperate,
