Re: [ccp4bb] weekend puzzle

2013-09-17 Thread Andreas Förster
Thanks to all who engaged with my little puzzle. The weekend is over and I'm not exactly closer to fitting the right pieces into the density. Benzamidine was a good suggestion for the first bit of density. However, I didn't use that inhibitor during purification. The second set of blobs does

Re: [ccp4bb] weekend puzzle

2013-09-13 Thread Paul Emsley
On 13/09/13 17:50, Andreas Förster wrote: Dear all, a structure I'm refining is enjoying a free R factor of 18 but still suffers from mystery density that nothing in the crystallization condition could explain. I can't explain it either. Maybe you like to venture some guesses? The missing

[ccp4bb] weekend puzzle

2013-09-13 Thread Andreas Förster
Dear all, a structure I'm refining is enjoying a free R factor of 18 but still suffers from mystery density that nothing in the crystallization condition could explain. I can't explain it either. Maybe you like to venture some guesses? The missing ligand is derivatized benzoic acid, nitrob