- Original Message
From: David Briggs drdavidcbri...@gmail.com
To: CCP4BB@JISCMAIL.AC.UK
Sent: Monday, 21 December, 2009 17:26:15
Subject: Re: [ccp4bb] where I have been going wrong in crystallization?
Hi Martyn,
this recipe tends to work for me...
Lysozyme: 50 mg/ml in 0.1 M Sodium
Hi Martyn,
When it comes to micro-dialysis, you could check the following paper:
http://www.jbc.org/content/260/25/13580.full.pdf+html
(freely available, check in particular the appendix for the
micro-dialysis setup that was used, that was for heat-labile enterotoxin)
Fred.
PS I know this
Dear All
checking out the Lysozyme crystallization methods on the web I liked the
Rigaku Instructions that I found:
(http://www.rigaku.com/protein/crystallization.html)
...create a drop of 3ul lysozyme solution, and 3 ul of well solution,
respectfully, for a total drop size of 6ul...
So
Hi Martyn,
this recipe tends to work for me...
Lysozyme: 50 mg/ml in 0.1 M Sodium Acetate pH 4.8
Reagent: 8% w/v Sodium Chloride, 0.1 M Sodium Acetate pH 4.8
Additional Reagents: Index Reagent 8, 22, 28, 31, 34, 40, 58, 59, 69, 86, 88
Mix equal amounts of lysozyme with reagent, incubate at 4 or
Dear Martin,
The original procedure with polyethylene glycol comes from:
Stura, E.A. (1998) Strategy 3: Reverse Screening. In Crystallization of
Proteins: Techniques, Strategies and Tips. A laboratory manual (Bergfors,
T., ed) International University Line. pp. 113-124.
If you follow the
I was recently testing out the 30% PEGMME 5k, 0.1M NaOAc pH 4.5, 1M
NaCl method mentioned in a Hampton Research catalog and attributed to
Enrico Stura. I see that he has also just commented on this thread.
I found that at 80mg/ml, batch with 1.5ul:1.5ul protein to precipitant
ratio,
- I think the original poster was only calling attention to the fact
that some proteins want to be treated respectfully in order to
crystallise (and the fact that Rigaku Japan realises this). I find
that indeed the case.
Other proteins, however, prefer the attitude I don't know why I am
and should be
explicit about such important ingredients.
Mark
-Original Message-
From: Mark J. van Raaij mark.vanra...@usc.es
To: CCP4BB@JISCMAIL.AC.UK
Sent: Mon, Dec 21, 2009 12:18 pm
Subject: Re: [ccp4bb] where I have been going wrong in crystallization?
- I think the original
recipes are indispensable and should
be explicit about such important ingredients.
Mark
-Original Message-
From: Mark J. van Raaij mark.vanra...@usc.es
To: CCP4BB@JISCMAIL.AC.UK
Sent: Mon, Dec 21, 2009 12:18 pm
Subject: Re: [ccp4bb] where I have been going wrong in crystallization
and
should be explicit about such important ingredients.
Mark
-Original Message-
From: Mark J. van Raaij mark.vanra...@usc.es
To: CCP4BB@JISCMAIL.AC.UK
Sent: Mon, Dec 21, 2009 12:18 pm
Subject: Re: [ccp4bb] where I have been going wrong in crystallization?
- I think the original
Maybe is a mistake by a non-English speaker. Instead of respectfully should
say
respectively
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