On Fri, 05 Feb 2010 05:39:14 +0100, rui ruis...@gmail.com wrote:
Hi, All,
We are trying to crystallize a protein and found some initial hit in the
following conditions,
pH 4.8, 0.2 M AS or some other salts ( NaCl,LiCl, MgCl2 ), 32% PEG4000 or
PEG3350 ). However the quality of the crystal is
by: CCP4 bulletin board CCP4BB@JISCMAIL.AC.UK
05-Feb-2010 09:48
Please respond to rui ruis...@gmail.com
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Re: [ccp4bb] how to improve resolution
Hi, Thanks all for the good suggestions, I just attached two images that
show my crystals, maybe you'll see new
My interpretation of hollow crystals is that the crystals are growing
too fast. I've had success with similar looking crystals by slowing
crystal growth. You can try lower temperature, protein concentration,
or precipitant. In my case, I found success by trying different
concentrations of NaCl in
My interpretation of hollow crystals is that the crystals are growing too
fast.
This is indeed true and a well known effect observed also in mineral
crystals,
namely that the edges grow fastest and the faces fail to fill (or the
crystals
are filled with disordered material. The examples are the
Hi,
The situation is somewhat like my case. Have you checked the purity level of
the protein in SDS PAGE. In that case you may try to introduce another
purification step.
regards
Debajyoti
On Fri, 05 Feb 2010 10:05:23 +0530 wrote
Hi, All,
We are trying to crystallize a protein
Hi Rui,
In addition to what Fred has advised, you could also try
varying other parameters like temperature and protein concentration. 32 %
is
a very high concentration of precipitant. Maybe you could try using a
bigger PEG (like PEG 8000), at a lower concentration. It worked for me
once.
