Re: [ccp4bb] problems about installation of mosflm701

Hi First off, you really shouldn't be installing mosflm version 7.0.1 - version 7.0.3 is the current one, and contains numerous bug fixes. The developers are likely to meet any problem queries relating to 7.0.1 with update to the current version first, then try again; if the problem

Re: [ccp4bb] problems about installation of mosflm701

You would also need to change the top line of the file so that it was something like #!/bin/bash -f, though I would be more inclined to use a proper traditional Bourne shell for this and use #!/bin/sh -f and use sets and exports throughout. That's not actually necessary for a 'sourced'

[ccp4bb] OMIT map from Sfcheck

Dear, I'm intending to make an OMIT map using Sfcheck (ccp4i 6.0.2, Mac OS X Tiger, PPC). However, the program terminates with: has failed with error message 1525-005 1525-005 1525-005 1525-005 1525-005 1525-005 When using 'command line' from a script (with '-nomit 2 -map -origin - out

[ccp4bb] expression vector

Hello,everyone! I have a question,is the expression vector effect crystallization much? For example,PQE2-protein structure has 2 molecules in one unit-cell,PQE70-protein single mutant has 5molecules in one unit-cell.Is the vector effect the results or the mutant itself? Can

[ccp4bb] Pointless 1.2.15 and ccp4i

Dear List, I am having a problem with running the latest version of pointless (1.2.15) via ccp4i. This is with the new GUI task. The logfile has the following, rather unhelpful information: SNIP *** * Information

Re: [ccp4bb] expression vector

杨柳青 wrote: Hello,everyone! I have a question,is the expression vector effect crystallization much? For example,PQE2-protein structure has 2 molecules in one unit-cell,PQE70-protein single mutant has 5molecules in one unit-cell.Is the vector effect the results or the mutant itself? Can

Re: [ccp4bb] Pointless 1.2.15 and ccp4i

I've had some problems getting recent versions of Pointless to link in a portable way, due to a new call into the CCP4 library, this can cause segfaults on some systems. I keep hoping someone will tel me how to get round this (a ccp4 routine calls getpwid which apparently cannot be static) You

Re: [ccp4bb] Pointless 1.2.15 and ccp4i

Installation of pointless-1.2.16 appears to have solved the problem. On Thu, 2008-03-27 at 14:55 +, Phil Evans wrote: I've had some problems getting recent versions of Pointless to link in a portable way, due to a new call into the CCP4 library, this can cause segfaults on some systems. I

Re: [ccp4bb] expression vector

I agree with Roger - provided, of course, that the same protein is expressed from either vector with no extra amino acids at either end. Quite often vectors include extra sequence at the N or C terminus that are remnants of regions or linkers between the protein and purification tags.

Re: [ccp4bb] expression vector

Hi,Yang, Tag(type and length) ,surface charge and crystallization condition are parameters which affect the packing in the crystal. It should be the tag or mutation not the the vector itself made you get different unit cell. ta liu 杨柳青 wrote: Hello,everyone! I have a question,is the expression

[ccp4bb] Placing an EM map in a large P1 box

Hi All, I'm having trouble putting an EM map into a large P1 box for molecular replacement. We're trying to put density for a molecule about 300A in size into a 1000A P1 box. Using maprot in cutting mode with a mask yields a map with 1000A axes, but the extent of the map only covers

[ccp4bb] about Mosflm once more

Hello, Thank you very much for your help, now I setup Mosflm703 successfully. By the way, can I remove the ccp4 program suite including previous version and integrate the latest version into ccp4. Thanks Sincerely Lu Yongzhi