Hi Zhijie --
With all manipulations on the goniometer, I strongly advise what I
learnt from Elspeth Garman: practice, practice, practice... but not
with your real crystal! Take it down, put up a dummy pin, and take the
time play around. I imagine even a non-hollow pin can be bent without
On 06/08/11 08:53, Joe Watts wrote:
I don't know about the Quadro 4000, but with the FX3800 I had a hell of a time
tracking down a 3 pin stereo bracket. I eventually was directed to a vendor
that sold a bracket by an engineer from NVIDIA. PNY Technologies PNQFX3800
Stereo Bracket for Quadro
On Wed, Jun 08, 2011 at 10:41:03AM -0400, Angela (Shaoxu) Li wrote:
I wish to unsubscribe to the mailing list. But I'm unsure as to how I
can do that. Your help will be greatly appreciated.
In the headers of every email sent from the list are some helpful
reminders:
List-Help:
Hi,
What about the ccp4 web page, from which you can follow the link to
http://www.ccp4.ac.uk/ccp4bb.php ?
HTH,
Fred.
Angela (Shaoxu) Li wrote:
Hi there,
I wish to unsubscribe to the mailing list. But I'm unsure as to how I
can do that. Your help will be greatly appreciated.
Best
Dear all,
I have a P2 derivative dataset with beta=89.6. I try to change the
beta to 90.4 to be consistent with the native dataset. Should I do sth
with the HKL, like applying a matrix? Thanks a million!
Best,
Zhiyi
Zhiyi Wei wrote:
Dear all,
I have a P2 derivative dataset with beta=89.6. I try to change the
beta to 90.4 to be consistent with the native dataset. Should I do sth
with the HKL, like applying a matrix? Thanks a million!
Best,
Zhiyi
Hi,
Personally I would use sftools (no ccp4i GUI), to
Hi Zhiyi,
This is very easily done using Pointless. From the gui, click Match index to
reference, enter your two mtz files and run.
ERic
Eric T. Larson, PhD
Biomolecular Structure Center
Department of Biochemistry
Box 357742
University of Washington
Seattle,
Thx Clemens, - my indexing gives the same setting, correct is -a -b c
BR
-Original Message-
From: Clemens Vonrhein [mailto:vonrh...@globalphasing.com]
Sent: Wednesday, June 08, 2011 9:06 AM
To: Bernhard Rupp (Hofkristallrat a.D.)
Subject: Re: [ccp4bb] Change cell parameter
Hi Bernhard,
you probably need to reindex your data.
h - h
k - -k
l - -l
by using the command
hkl matrix
1 0 0
0 -1 0
0 0 -1
in scalepack
In HKL2000 you should use reindex menu or data set macro (not the overall
scaling macro). Dataset macro exists only in the newest version of
HKL2000.
The reindexing
Thx Clemens, - my indexing gives the same setting, correct is -a -b c
BR
-Original Message-
From: Clemens Vonrhein [mailto:vonrh...@globalphasing.com]
Sent: Wednesday, June 08, 2011 9:06 AM
To: Bernhard Rupp (Hofkristallrat a.D.)
Subject: Re: [ccp4bb] Change cell parameter
Hi Bernhard,
Dear All,
I am working on a protein structure that yielded comparable diffraction
quality crystals from two different crystallization condition. One of the
crystallization condition conatins high conc. of salt pptant whereas the
oher one contains high conc. of organic pptant. There are some
Reindex your data as -h -k l or h -k -l - this will automatically
change the cell to berta = 90.4
eleanor
On 06/08/2011 04:10 PM, Vellieux Frederic wrote:
Zhiyi Wei wrote:
Dear all,
I have a P2 derivative dataset with beta=89.6. I try to change the
beta to 90.4 to be consistent with
Dear all,
just to show what I mean where the problem is: I've produced 1 million
Gaussian random numbers with a mean of 1 and a standard deviation of 1.
I attach a plot showing both the Gaussian itself, and the distribution
of the numbers obtained by taking the inverse. The latter looks quite
Dear Users,
Please note: Due to an extended shutdown, proposals will not be solicited
in May for the July-August 2011 beamtime. The next cycle will be
September-October 2011. The deadline to submit proposals will be July 15th.
Regards
Banu
HI,
I am a beginer in crystallography. I have carried out
molecular replacement in 'phenix' and now want to refine the structure
in ccp4. Could anyone tell me what the proper Rfree_flag value to put
in while using Refmac5(ccp4). I have used 1 and output seems to be
fine but I want
On Wed, Jun 8, 2011 at 4:44 PM, Madhu shankar Madhu shankar
mad07...@student.otago.ac.nz wrote:
I am a beginer in crystallography. I have carried out molecular
replacement in 'phenix' and now want to refine the structure in ccp4. Could
anyone tell me what the proper Rfree_flag value to
Dear Shiva,
It has happened many times. This paper (not about crystallography) is one
example:
http://onlinelibrary.wiley.com/doi/10./j.1742-4658.2005.05047.x/abstract;jsessionid=864D79CC1089210C6A3CDDAC3AFE25DB.d03t04
Why don't you try SEC with high salt concentration?
Yours,
Clement
On
Dear all,
Thank you all! There are so many useful replies. The problem has been
solved by using the way introduced by Clemens (see below, I dont have
an access to SCALEPACK).
BTW, although beta is close to 90, it is not a orthorhombic crystal
(Rmerge goes up to ~0.3 after scaling).
Thanks
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