Dear all,
We have an open position for a structural biologist with strong background both
in NMR and X-ray crystallography, for our team at Bayer in Berlin. All details
can be found in the job advertisement under the following link:
Thanks for all suggestions. I am going to P32-label the RNA and see if it
runs as a single species on the gel and if not, I'll do the HPLC.
Alex
On Mon, May 20, 2013 at 11:16 AM, Eugene Valkov eugene.val...@gmail.comwrote:
Hi Alex,
If you do not have access to HPLC equipment, another
hi all,
Sorry if this has been asked before. I wonder if there is an list or library of
most commonly co-crystallized ligands(or solvent molecules) available? Better
if the electron density maps of the ligands are also shown with different
resolutions. That could help a lot for an
Hi Jeremy,
Given the large number of different kinds of ligands that are observed (either
from crystallization, purification conditions or endogenous), I think it will
be really difficult to assign just by visual and manual comparison with a
standard set, especially for inexperienced
Top 20 HETNAM entries based on 58,469 PDB entries at better than 2.5 Angstrom
resolution (arbitrary cut):
Number of entries in histogram: 14864
Total number of instances : 195481
0 14502 0.0742 GOL(glycerol)
1 10952 0.0560 SO4
2 8064 0.0413 ZN
3 7628 0.0390 MG
4 6930
Does anyone have a script to convert pdb file with DNA atom records from
v3 back to v2? I can certainly right my own and asking only if you
already have it written. Strictly speaking, this is not ccp4-related -
apparently, buster expects the old format.
Cheers,
Ed.
--
Oh, suddenly
On 05/21/2013 04:35 PM, Francis Reyes wrote:
Since you're using buster, have you tried global phasing's own pdbvconv tool?
Naturally, but it leaves file unchanged.
--
Oh, suddenly throwing a giraffe into a volcano to make water is crazy?
Julian,
I'm not sure that a library of ligand electron density would be an entirely
good thing. I guess something small like an acetate or DMSO would be
relatively consistent but larger things like PEGS or even glycerols are
going to be dependent on the nature of the binding site. A good example
would be
