[ccp4bb] PhD Position at Freie Universität Berlin

3-year PhD position at the Freie Universität Berlin A joint PhD position is available in the labs of Markus Wahl and Sutapa Chakrabarti (at the Institute of Chemistry and Biochemistry, Freie Universität Berlin) to work on structural and biochemical aspects of proteins involved in eukaryotic

[ccp4bb] Reminder - Travel bursaries for CCP4 Study Weekend 2019 - deadline for applications this Wednesday

Applications for travel bursaries to assist students and young post-docs at non-UK labs to attend the CCP4 SW need to be in by close of play this Wednesday. Not many applications received so far so get them in ASAP if you want to stand a chance! Regards Karen McIntyre Science & Technology

Re: [ccp4bb] suggestions for cryoprotectant

Hi Firdous, I had success last week with a condition containing 1.5M MgCl2 which I’d tried cryo-ing with high salt before and got no diffraction. Adding dimethylsulphoxide to a final concentration of 10% v/v in the well solution worked well for me. DMSO was bound in the structure which can be a

Re: [ccp4bb] suggestions for cryoprotectant

Condition #3 isn’t far from the saturated conditions for lithium sulfate. Perhaps slightly increasing the lithium sulfate concentration will work, or using a related lithium salt. See Cryosalts: suppression of ice formation in macromolecular crystallography Acta Cryst D Volume

[ccp4bb] PostDoc in Stockholm

Dear colleagues, I would like to advertise a position in cryoEM in Stockholm PostDoc in structure biology of ion channels Project description The project focuses on the purification and characterization of a ligand-gated ion channel. The candidate will characterize the appropriate expression

Re: [ccp4bb] suggestions for cryoprotectant

Firdous, Many crystallographers (if not all) went through case like you describe. So far you’ve got great suggestions that are worth trying. I’ll add one more from my own experience. In all cases I tried it appeared that addition of 0.5 to 1% v/v of glycerol or ethylene glycol to drop (either

Re: [ccp4bb] suggestions for cryoprotectant

Hi Firdous, Dry mineral oil usually works well, and it does not alter the crystallisation drop composition. Even better if you are using sitting drop. Just add enough mineral oil to cover the crystallisation drop, and you even have a lot more time to pull your crystal out before the high

[ccp4bb] Accepting General User and Training Proposals to the National Center for CryoEM Access and Training (NCCAT) - NYC, USA

Dear all, Due to the success of our early access program, the National Center for CryoEM Access and Training (NCCAT; nccat.nysbc.org) announces the opening of additional access categories. The General User Proposal (GUP) program allows researchers to use NCCAT resources

Re: [ccp4bb] [EXTERNAL] [ccp4bb] suggestions for cryoprotectant

Hi Firdous, What Diana suggested may work if done carefully; however, I failed to transfer crystals from 1.5 LiSO4 salts to higher concentrations of LiSO4 in many attempts. I did have success in transferring said crystals to 1.5 M sodium malonate then to incrementally to 2.8 or 3.2 M sodium