Dear Jon,
when you place an atom at the screen pointer, there is no symmetry that Coot
would be able to use. You need to tell Coot. One way is to merge it with an
existing molecule that has a valid symmetry description. Another way is to
create a separate PDB file with the symmetry of your
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University of Illinois at Chicago
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Many thanks to all who replied. The best scheme seems to be to add the new
waters into the main molecule and then make use of the options to renumber them
and change the chain ID later on. This has the added advantage of avoiding the
situation where, if you are not careful with the 'Place atom
Sorry for the off-topic post; only the first two out of the four positions
below are related to structural work, I've included the other two for
completeness' sake.
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- Principal Scientist, Discovery Chemistry
Dear experts,
I am trying to insert a non-natural nucleotide (5CM, methylated cytosine) at
6th position in a 13-mer DNA. I have generated the CIF file for 5CM using ELBOW
(Phenix). I tried replacing the Cytosine with 5CM and linking the O3' of -1
nucleotide with P of 5CM and o3' of 5CM with P
