Re: [ccp4bb] AW: [ccp4bb] [EXTERNAL] Re: [ccp4bb] number of frames to get a full dataset?

2020-07-01 Thread Kay Diederichs
Dear Dirk, one cannot fully correct radiation damage. Normal scaling procedures take care of the average decay by a smooth resolution-dependant function. Zero-dose extrapolation goes beyond that but needs all symmetry mates - this does not fulfill your definition of "identical". If we really

[ccp4bb] Cryo-EM beamline scientist permanent position at CBI / IGBMC, Strasbourg

2020-07-01 Thread Bruno KLAHOLZ
Dear all, here is an announcement for a permanent position as cryo-EM beamline scientist at the Centre for Integrative Biology, Strasbourg, France. Deadline is tomorrow 2.7.2020 12 am CET. With best regards, Bruno Klaholz Cryo-EM beamline scientist (permanent position): The IGBMC/CBI is

Re: [ccp4bb] AW: [ccp4bb] [EXTERNAL] Re: [ccp4bb] number of frames to get a full dataset?

2020-07-01 Thread Dirk Kostrewa
Dear Gerard and Kay, yes, you are both right - I have totally forgotten radiation damage! And correcting for this really makes a difference! However, if radiation damage is corrected for reflections measured at different time points under the same geometry, does anything speak against it,

[ccp4bb] Beamline scientist position - MX3 beamline at the Australian Synchrotron. [SEC=UNCLASSIFIED]

2020-07-01 Thread CARADOC-DAVIES, Tom
Hi All, We need a new beamline scientist in the MX team at the Australian Synchrotron in Melbourne. This position is on the new microfocus MX3 beamline which is currently being designed and is scheduled for first users July 2023. This is a new 4-year fixed-term position in the MX3 build team.

[ccp4bb] Open position in biophysics/structural biology at Merck Healthcare KGaA

2020-07-01 Thread Djordje Musil
Dear colleagues, There is an open position in biophysics/structural biology at Merck Healthcare KGaA in Darmstadt, Germany: https://jobs.vibrantm.com/merck/job/Darmstadt-Scientist-(all-genders)-HE-64293/605907601/?locale=de_DE Could you please forward the link to the colleagues you think might

Re: [ccp4bb] AW: [ccp4bb] [EXTERNAL] Re: [ccp4bb] number of frames to get a full dataset?

2020-07-01 Thread Dirk Kostrewa
Dear Kay & Gerard, the only reason, why I want to count differently, is to distinguish between true and pseudo-multiplicity. Apparently, I get on thin ice by trying to define "identical" reflections ... maybe, instead, we should start working with unmerged data in all programs. If I remember

Re: [ccp4bb] [EXTERNAL] Re: [ccp4bb] number of frames to get a full dataset?

2020-07-01 Thread Goldman, Adrian
I find, when discussing definitions of words, it’s always good to look in the OED (well, the SOED, I don’t have the big one). For redundant (redundancy being defined as the state or quality of being redundant), we find: 1. Superabundant, superfluous, excessive. b. Characterised by superfluity

Re: [ccp4bb] [EXTERNAL] Re: [ccp4bb] number of frames to get a full dataset?

2020-07-01 Thread Ian Tickle
Yes this seems to be a common misunderstanding, that the meanings of words such as 'redundancy' have to be the same in an informal non-scientific context and in a formal technical/scientific context. So we can say that in an informal context, 'redundancy' means "unnecessary duplication (or

[ccp4bb] AW: [ccp4bb] [EXTERNAL] Re: [ccp4bb] number of frames to get a full dataset?

2020-07-01 Thread Hughes, Jonathan
hi ian, oh no! all those trump fans across the pond will love the "hypothesis of evolution" idea. they won't know the word "hypothesis" of course, but unfortunately you might get famous for it anyhow. cheers jon Von: CCP4 bulletin board Im Auftrag von Ian Tickle Gesendet: Mittwoch, 1. Juli

Re: [ccp4bb] [EXTERNAL] Re: [ccp4bb] number of frames to get a full dataset?

2020-07-01 Thread John R Helliwell
Dear Ian, I take issue with your assertion below that “the totally precise **scientific** meaningis an **engineering**” definition. Science and engineering are not the same. Health and safety leads to the need in engineering for redundancy and indeed safety factors. In essence, in

[ccp4bb] Two Group Leader positions available@EMBL Hamburg

2020-07-01 Thread Margret Fischer
Dear colleagues, I would like to inform you that EMBL Hamburg is currently advertising for two Group Leader positions. *Reference Number HH00190*: Group leader - New approaches in electron microscopy /Closing date: 13th August 2020/ *Reference Number HH00191:* Group Leader - X-ray imaging

Re: [ccp4bb] number of frames to get a full dataset?

2020-07-01 Thread James Holton
Sorry to take this thread on a detour/diversion: What I was attempting to point out below, perhaps unclearly, is that the different interpretations of the word "redundant" are a cultural difference.  As a student of multiple English languages perhaps I can explain: Few US English speakers

Re: [ccp4bb] number of frames to get a full dataset?

2020-07-01 Thread jp d
since i am not getting shit/shite done may i also point out aluminium/aluminum On Wednesday, July 1, 2020, 11:52:21 AM PDT, James Holton wrote: Sorry to take this thread on a detour/diversion: What I was attempting to point out below, perhaps unclearly, is that the different

[ccp4bb] Postdoc position in the Fesik Lab at Vanderbilt University

2020-07-01 Thread Phan, Jason
Please post: Advertisement for a postdoctoral structural biologist position at Vanderbilt University Medical Center, Department of Biochemistry A postdoc position is available in the laboratory of Dr. Stephen Fesik

Re: [ccp4bb] [EXTERNAL] Re: [ccp4bb] number of frames to get a full dataset?

2020-07-01 Thread Jose Brandao-Neto
Hi Ian, good to hear! Hi everyone, thanks for the etymological - and etiological - discussion. I'm good whatever the choice. John, I beg to differ with the absolute statement that xfels offer damage free hkls - back in 2016 yet another great experimental work, by Inoue et al

Re: [ccp4bb] [EXTERNAL] Re: [ccp4bb] number of frames to get a full dataset?

2020-07-01 Thread Ethan A Merritt
On Wednesday, 1 July 2020 18:50:57 PDT Jose Brandao-Neto wrote: > Hi Ian, good to hear! Hi everyone, thanks for the etymological - and > etiological - discussion. I'm good whatever the choice. > > John, I beg to differ with the absolute statement that xfels offer damage > free hkls - back in

[ccp4bb] PostDoctoral Position in Membrane Protein Structural Biology at USC, Los Angeles

2020-07-01 Thread Cornelius Gati
PostDoctoral Position in Membrane Protein Structural Biology at USC, Los Angeles The position is available starting January 2021 in the Department of Biological Sciences at the University of Southern California and is initially limited to 2 years, with possible extension. Our research focuses

[ccp4bb] AW: [ccp4bb] [EXTERNAL] Re: [ccp4bb] number of frames to get a full dataset?

2020-07-01 Thread Schreuder, Herman /DE
Dear Bernard and other bulletin board members, As Gerard mentioned, current data processing programs and table 1’s do not make this distinction, but of course, you are free to ask the community to introduce it. My proposal to use “measurements per reflections” is not a joke. It exactly

[ccp4bb] AW: [ccp4bb] AW: [ccp4bb] [EXTERNAL] Re: [ccp4bb] number of frames to get a full dataset?

2020-07-01 Thread Schreuder, Herman /DE
Dear Frank, in general it is not possible to determine the intensity of a reflection from a single fine slice. One needs slices for the complete reflection. Also, like Bernard, you are imposing criteria on the MPR, which are not imposed on the multiplicity/redundancy/abundancy. All I ask the

Re: [ccp4bb] [EXTERNAL] Re: [ccp4bb] number of frames to get a full dataset?

2020-07-01 Thread John R Helliwell
Good morning Jon, Ah yes that is a good word from quantum mechanics but no it isn’t in the IUCr Dictionary, nor in the Statistical Descriptors section on Recommendations. http://ww1.iucr.org/iucr-top/comm/cnom/statdes/recomm.html In Laue mode, Xray or neutron, the MPR should be large enough to

Re: [ccp4bb] AW: [ccp4bb] [EXTERNAL] Re: [ccp4bb] number of frames to get a full dataset?

2020-07-01 Thread Dirk Kostrewa
Dear Herman, I think, your MPR proposal is a great idea and would like to second it! And I would also like to propose that data processing programs just average "identical" reflections measured under the same geometry and count them only once (*), so that, in the end, we will get a realistic

Re: [ccp4bb] AW: [ccp4bb] [EXTERNAL] Re: [ccp4bb] number of frames to get a full dataset?

2020-07-01 Thread Gerard Bricogne
Dear Dirk, Aren't you for getting about radiation damage? The n measurements of the same hkl with the same geometry would not be equivalent, although they would enable the tracking of radiation damage without the confounding with absorption effects that comes from considering

Re: [ccp4bb] AW: [ccp4bb] [EXTERNAL] Re: [ccp4bb] number of frames to get a full dataset?

2020-07-01 Thread Kay Diederichs
Dear Dirk, XDS_ASCII.HKL (and equivalent files from other processing software) gives you all the information that you're after, since every reflection is stored individually. However when you analyze that, you will find that in a data set that comprises less than 360 degrees of rotation, there