Dear CCP4BB
PDB data will shortly incorporate a new data representation for
carbohydrates in PDB entries and reference data that improves the
Findability and Interoperability of these molecules in macromolecular
structures. In order to remediate and improve the representation of
carbohydrates
Hi there,
*Two postdoctoral fellow* positions are immediately available in Tianmin
Fu’s laboratory in the Department of Biological Chemistry and Pharmacology
at the Ohio State University. The positions are supported by NIH and
departmental funding to study molecular mechanism of signaling protein
Dear Antony,
similar to the approach Eleanor has described, lsqman will give you
rotational matrix and translation vector.
http://xray.bmc.uu.se/usf/lsqman_man.html
Best regards, Guenter
Hi,
You can try elNemo server.
Regards,
Nasrin
PhD scholar
On Wed, Jul 8, 2020, 10:16 PM Antony
Hi Folks, how are you doing? Hope everyone is safe!
I was wondering if there is a bulletin board email group like this one
concerning Cell Biology, where we could find not only discussions about related
topics but also post-doc opportunities. I would be very glad if you guys know
any and could
Hi Umar,
I must say that it would be better use as an expression system Rosetta DE3 or
Rosetta-gami instead of BL21. In our lab we generally use BL21 for plasmid
replication.
Also, there are a few things that you could try before trying another
construction.
1. Lower the temperature
Re: "In our lab we generally use BL21 for plasmid replication."I am interested because on the occasions that I prepared plasmids in BL21 (usually by mistake), they were never any good for cloning or sequencing. I always had to transform them back into a cloning strain and do another plasmid prep!
