[ccp4bb] 3 letter code for the Ligand

Hi, 1) I am working with two ligands, cyclopropanemethanol and cyclobutanemethanol. The 3 letter code for them CPM and CBM (i thought of using) were assigned to other ligands. Is there any standard procedurre to name them. I would like them to use in Refmac and coot. 2) How do I convert mtz file

Re: [ccp4bb] Fancy crystal, poor diffraction

>> Have you tried DTT (or another way of keeping things in a reducing >> environment)? This might help keep your disulfides (and hopefully >> protein) happy. > > > Surely the addition of a _reducing_ agent would _reduce_ your disulphides > - > i.e. break them. Whoops...sorry, I guess I've got zin

Re: [ccp4bb] conversion of .scl file to .mtz file

The Automar manual claims that .scl is scalepack format, so just give it a try and check with mtzdmp that the converted values make sense (maxima, minima of h,k,l, I, sigma(I)). -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A On Thu, 25

Re: [ccp4bb] relation between wavelength and inter-atomic distances

Diana Tomchick wrote: The most conceptually simple example of diffraction is single-slit diffraction in which the slit is narrow, that is, significantly smaller than a wavelength of the wave. What ? Diffraction when the slit is much smaller than the wavelength ??? After the wave passes throug

[ccp4bb] conversion of .scl file to .mtz file

hi all i have scaled files in .scl format, obtained by automar. How can I convert .scl file into .mtz file (CCP4 format)? Although in CCP4 there is an option scalepack2mtz but it uses .sca format as an input . Is .sca (denzo) format is same as .scl format? thanks in advance vineet

Re: [ccp4bb] Fancy crystal, poor diffraction

In case that the poor diffraction quality is a result of problems with flash cooling directly into liquid nitrogen, it may also be worth trying to remove the cold gas layer above the liquid nitrogen prior to dipping the crystal: Warkentin et al.: "Hyperquenching for protein crystals", J. Appl.

Re: [ccp4bb] Fancy crystal, poor diffraction

On 24/01/07, Peter Adrian Meyer <[EMAIL PROTECTED]> wrote: > We have crystallized a 21KD protein with 2 disulfide bonds grown for one > month in 0.1M tri-sodium citrate pH 5.6, 0.5M (NH4)2SO4 and 1M Li2SO4. The > crystals look big (~0.4mm x 0.4mm x 0.3mm) and pretty (sharp edge, clean > surface)

[ccp4bb] [Fwd: [ccp4bb] relation between wavelength and inter-atomic distances]

Carlos Frazao wrote: Hi, I have once heard and recently read that "the diffraction event results from the fact that both the X-rays wavelength and the atomic distances are of the same magnitude". Although such a relation seems appealing I am unsure if this is not a mere coincidence. Could som