[ccp4bb] mlphare ref

Dear All, I failed to find a specific reference or description of mlphare in the prog doc. I found some ref to Otwinowski 1991 Proc CCP4 study weekend pp 80-86 Does someone perhaps have this ref in pdf or another suggestion? Thx, br Bernhard Rupp

Re: [ccp4bb] TFT monitors n stereo

Hi, we have just gotten some relatively inexpensive E-dimensional glasses (cable controlled; IR versions available) to test. Using their Windows driver, supposedly working on both ATI and NVIDIA, hardware stereo on my TFT worked. Flicker would be a problem for many people, however. I have yet to

[ccp4bb] heavy metal labelled GTP derivatives

Does anyone know of any heavy metal labelled GTP derivatives and if so where to get them? thanks Stephen -- ** Dr. Stephen Cusack, Head of Grenoble Outstation of EMBL Group leader in structural biology of protein-RNA

Re: [ccp4bb] heavy metal labelled GTP derivatives

Hi Stephen, you can get Iodinated or Brominated nucleotide derivatives from jena bioscience (http://www.jenabioscience.com/cms/en/1/browse/106_nucleotides_and_their_analogs.html) hth, Sebastiano At 02:44 PM 5/7/2007 +0200, Stephen Cusack wrote: Does anyone know of any heavy metal labelled GTP

[ccp4bb] Metal analysis in proteins

Can anyone recommend a facility that analyzes the metal content of proteins by ICP or some other technique? Thanks. Jack Tanner --- John J. Tanner Professor of Chemistry and Biochemistry University of Missouri-Columbia 125 Chemistry Building 601

Re: [ccp4bb] heavy metal labelled GTP derivatives

Another option might be to bind Hg to a phosphorothioate GTP (I've never actually tried it). Stephen Cusack wrote: Does anyone know of any heavy metal labelled GTP derivatives and if so where to get them? thanks Stephen --

[ccp4bb] Problem on creating a new monomer

Hi there, I am trying to build a new ligand that I need to put into my structure. The problem I have right now is that, after regularising the molecule, a six-member ring always flip to the opposite orientation (the electron density map is defintely telling me which way the six-membered ring

Re: [ccp4bb] Problem on creating a new monomer

Hi XJXJ, Did you create a library for this yourself? If you fix the ligand into the density and use those coordinates to create a monomer library for it in refmac, and use this during refinement, it might let it stay that way. Arti Hi there, I am trying to build a new ligand that I need to put

Re: [ccp4bb] Alternate space groups for MR in Beast

Ed, I would highly recommend Phaser to do what you want. Phaser can be configured to search all possible related space groups, or the ones you designate. Open-EPMR can also do automated related space-group searching as well. Cheers, ___ Roger S. Rowlett

[ccp4bb] SAM phasing

Hello all, Does anyone know where I can obtain heavy atom-derivatized S-adenosylmethionine? I am wondering if anyone has experienced or heard of successful instances where heavy atom-derivatized SAM has been used for phasing molecules that binds SAM? Thanks a lot! Ray

[ccp4bb] Thanks! Re: [ccp4bb] Problem on creating a new monomer

Thanks for all the quick replies! The problem turned out to be that the Turbo output pdb file of my ligand (created by using the MAKE command) somehow failed to label all my carbon atoms as C. Instead, all my carbons were labelled with just the number. For example, C12 was mislabelled as 12.