Re: [ccp4bb] Sulfate ion on 2-fold axis
Using SHELXL, restraints can be applied to symmetry equivalent atoms so one can easily restrain the sulphate to be a regular tetrahedron by restraining the S-O distances to be equal and the O1..O2, O1'..O2 etc. distances to be equal (with or without target values). For details of a similar example see Acta Cryst. A64 (2008) 112-122. From Eleanor's comment it appears that REFMAC can now do this too, though I was not aware of the facility. If you then wish to refine S anisotropically SHELXL will also automatically generate and apply the constraints required for the special position (in any space group), this can be very useful for high-resolution refinements. George Prof. George M. Sheldrick FRS Dept. Structural Chemistry, University of Goettingen, Tammannstr. 4, D37077 Goettingen, Germany Tel. +49-551-39-3021 or -3068 Fax. +49-551-39-2582 On Fri, 11 Jan 2008, Charlie Bond wrote: Eleanor Dodson wrote: It should be more or less equivalent, but better I think to put 1S at 0.5 occ and 2O at occ = 1 At least in REFMAC the restraints to the symmetry atoms should be set up But if you do that, some of the bond angles will be unrestrained, won't they?: O1-S-O2 will be OK, but O1-S-O1' and O2-S-O2' will not. Or is refmac cleverer than I think? Cheers, Charlie -- Charlie Bond Professorial Fellow University of Western Australia School of Biomedical, Biomolecular and Chemical Sciences M310 35 Stirling Highway Crawley WA 6009 Australia [EMAIL PROTECTED] +61 8 6488 4406
[ccp4bb] Multiple Signature Motifs in a single protein
Dear sir, I'm in something of a fix as I need to compile a dataset of proteins to test my work. The dataset would need to consist of proteins that contain multiple signature motifs. I am having difficulty finding any with more than two signature motifs. I would be grateful if you can let me know of any proteins that have multiple (three or more) signature motifs. Thank you in advance. Yours sincerely, Bioinformatics Centre Supercomputer Education and Research Centre Indian Institute of Science Bangalore - 560 012 E-mail : [EMAIL PROTECTED] Tel : 080-22932469 Ext:28 -- This message has been scanned for viruses and dangerous content by MailScanner, and is believed to be clean. -- This message has been scanned for viruses and dangerous content by MailScanner, and is believed to be clean.
Re: [ccp4bb] scale data with multiple MTZ files
There is an (possibly) easier way now in pre-release 1) Download install (manually) from the CCP4 pre-release site the program pointless and the ccp4i task interface 2) Use the Find or Match Laue Group option (under Data Reduction) to open the interface window to the program Pointless This will allow input of multiple MTZ files (or indeed files from XDS Scalepack), check them for consistent indexing if appropriate, and enforce unique batch numbers (a long-standing irritation). By default it will try to determine the Laue group space group, but it can also match space group indexing with a reference file, or just sort the files together (replacing sortmtz). 3) Put the output (HKLOUT) file into Scala (Scale and Merge intensities task) Note that Pointless ( Scala) are still (!) under development, and the versions in the next CCP4 release (or indeed pre-release) will be updates of these versions. Latest versions of Pointless Scala are also available from ftp://ftp.mrc-lmb.cam.ac.uk/pub/pre/ Phil On 11 Jan 2008, at 05:40, Roger Rowlett wrote: Huiying Li wrote: I tried to scale, using SCALA through CCP4i GUI, three blocks of data collected with one crystal (3 mtz files output from MOSFLM). The GUI has only one MTZ input slot. Which program can be used to combine the 3 unmerged mtz files together? CAD refused to handle these raw mtz files. Thanks in advance for any help. Huiying Using the CCP4 GUI, employ the following steps: 1. Open a Sort/Modify/Combine job and renumber your data sets (reset batch numbers option). A simple method is to add 1000 to the batch numbers for one data set and 2000 to the batch numbers for the second data set (assuming you have less than 999 frames of data output from MOSFLM.) 2. Open another Sort/Modify/Combine job and combine the renumbered MTZ files into one merged file. Click on Add File to add additional renumbered batches from step 1. Each batch of reflections will now have unique batch numbers. 3. Open a Scale and Merge Intensities task window. Select as your input the sorted and combined file output from step 2. In the Define Datasets section, choose Combine All Input Datasets into a Single Output Dataset. You can convert the scaled intensities to structure factors by checking the appropriate box. The combined datasets will not have monotonically varying R(merge) values across batches (1-1000, 1001-2000, 2001-3000) becuase of discontinuities in the data. However the merged datasets should have good overall statistics if appropriate for merging. Cheers, -- Roger S. Rowlett Professor Colgate University Presidential Scholar Department of Chemistry Colgate University 13 Oak Drive Hamilton, NY 13346 tel: (315)-228-7245 ofc: (315)-228-7395 fax: (315)-228-7935 email: [EMAIL PROTECTED]
Re: [ccp4bb] scale data with multiple MTZ files
Hi There's a way to avoid having to do this - use the ADD sub-keyword when processing in Mosflm, so that the batch number for each image data is given an offset; see http://www.mrc-lmb.cam.ac.uk/harry/cgi-bin/keyword2.cgi?PROCESS Both the traditional X11 gui and the new iMosflm allow you to add an offset easily, allowing the subsequent CCP4 programs to work with multiple MTZ files produced by Mosflm. On 11 Jan 2008, at 09:49, Phil Evans wrote: There is an (possibly) easier way now in pre-release 1) Download install (manually) from the CCP4 pre-release site the program pointless and the ccp4i task interface 2) Use the Find or Match Laue Group option (under Data Reduction) to open the interface window to the program Pointless This will allow input of multiple MTZ files (or indeed files from XDS Scalepack), check them for consistent indexing if appropriate, and enforce unique batch numbers (a long-standing irritation). By default it will try to determine the Laue group space group, but it can also match space group indexing with a reference file, or just sort the files together (replacing sortmtz). 3) Put the output (HKLOUT) file into Scala (Scale and Merge intensities task) Note that Pointless ( Scala) are still (!) under development, and the versions in the next CCP4 release (or indeed pre-release) will be updates of these versions. Latest versions of Pointless Scala are also available from ftp://ftp.mrc-lmb.cam.ac.uk/pub/pre/ Phil On 11 Jan 2008, at 05:40, Roger Rowlett wrote: Huiying Li wrote: I tried to scale, using SCALA through CCP4i GUI, three blocks of data collected with one crystal (3 mtz files output from MOSFLM). The GUI has only one MTZ input slot. Which program can be used to combine the 3 unmerged mtz files together? CAD refused to handle these raw mtz files. Thanks in advance for any help. Huiying Using the CCP4 GUI, employ the following steps: 1. Open a Sort/Modify/Combine job and renumber your data sets (reset batch numbers option). A simple method is to add 1000 to the batch numbers for one data set and 2000 to the batch numbers for the second data set (assuming you have less than 999 frames of data output from MOSFLM.) 2. Open another Sort/Modify/Combine job and combine the renumbered MTZ files into one merged file. Click on Add File to add additional renumbered batches from step 1. Each batch of reflections will now have unique batch numbers. 3. Open a Scale and Merge Intensities task window. Select as your input the sorted and combined file output from step 2. In the Define Datasets section, choose Combine All Input Datasets into a Single Output Dataset. You can convert the scaled intensities to structure factors by checking the appropriate box. The combined datasets will not have monotonically varying R(merge) values across batches (1-1000, 1001-2000, 2001-3000) becuase of discontinuities in the data. However the merged datasets should have good overall statistics if appropriate for merging. Cheers, -- - --- Roger S. Rowlett Professor Colgate University Presidential Scholar Department of Chemistry Colgate University 13 Oak Drive Hamilton, NY 13346 tel: (315)-228-7245 ofc: (315)-228-7395 fax: (315)-228-7935 email: [EMAIL PROTECTED] Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, MRC Centre, Hills Road, Cambridge, CB2 2QH Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, MRC Centre, Hills Road, Cambridge, CB2 2QH
Re: [ccp4bb] scale data with multiple MTZ files
pointless now does this Phil On 11 Jan 2008, at 11:05, Frank von Delft wrote: There's a way to avoid having to do this - use the ADD sub- keyword when processing in Mosflm, so that the batch number for each image data is given an offset; see Nevertheless, it *is* a right-royal pain in the arse: you're cruising at the synchrotron, crystal collecting and quietly (or noisily) dying, you want a quick scale to know whether you have enough data from your second batch and scala craps at you for having the repeating batch number. Can't the merging process (pointless; sortmtz) be made to spot that batch numbers are repeating, and do an automatic add based on filename? After all, batches from the same range *always* end up in the same unmerged mtz file (or files with the same template). At least, I was able to write a csh script to do it, and if you can do it in csh, you can do it in anything... :) phx.
Re: [ccp4bb] scale data with multiple MTZ files
There's a way to avoid having to do this - use the ADD sub-keyword when processing in Mosflm, so that the batch number for each image data is given an offset; see Nevertheless, it *is* a right-royal pain in the arse: you're cruising at the synchrotron, crystal collecting and quietly (or noisily) dying, you want a quick scale to know whether you have enough data from your second batch and scala craps at you for having the repeating batch number. Can't the merging process (pointless; sortmtz) be made to spot that batch numbers are repeating, and do an automatic add based on filename? After all, batches from the same range *always* end up in the same unmerged mtz file (or files with the same template). At least, I was able to write a csh script to do it, and if you can do it in csh, you can do it in anything... :) phx.
Re: [ccp4bb] scale data with multiple MTZ files
The task - Sort/ Modify / Combine files does just that.. Eleanor Huiying Li wrote: I tried to scale, using SCALA through CCP4i GUI, three blocks of data collected with one crystal (3 mtz files output from MOSFLM). The GUI has only one MTZ input slot. Which program can be used to combine the 3 unmerged mtz files together? CAD refused to handle these raw mtz files. Thanks in advance for any help. Huiying
Re: [ccp4bb] Sulfate ion on 2-fold axis - Garib?
It needs Garib to answer this! Eleanor Charlie Bond wrote: Eleanor Dodson wrote: It should be more or less equivalent, but better I think to put 1S at 0.5 occ and 2O at occ = 1 At least in REFMAC the restraints to the symmetry atoms should be set up But if you do that, some of the bond angles will be unrestrained, won't they?: O1-S-O2 will be OK, but O1-S-O1' and O2-S-O2' will not. Or is refmac cleverer than I think? Cheers, Charlie
Re: [ccp4bb] Coot for Fedora Core 8?
Hi Fred, we use this version and we did not have any problem. http://www.ysbl.york.ac.uk/~emsley/software/binaries/nightlies/pre-release/coot-0.4-pre-2-revision-618-binary-Linux-i386-redhat-8.0.tar.gz We usually use redhat-8.0 binary versions. Best wishes! Petr Kolenko Vellieux wrote: hi, The subject tells it all. Is there somewhere a version of Coot that is suitable for Fedora Core 8? Thanks in advance, Fred.
[ccp4bb] Postdoc position at Groningen University
I am posting on behalf of Dr. Dirk Slotboom, which has a job opening for a postdoctoral researcher. Post doc position in protein crystallography A 3-year postdoctoral position is available to study the structure of membrane transporters by X-ray crystallography. The position is part of a European Framework 7 program aimed at the structural elucidation of transporters and channels. The project aims at the elucidation of the molecular mechanism of ABC (ATP binding cassette) transporters involved in osmoregulation and multidrug transport (e.g. PNAS USA, 2006, 103:10624). The ABC transporters to be studied have been overexpressed already in our lab and can be purified in large quantities (e.g. Nature Meth., 2007 4: 705) Therefore, the project is ready for immediate crystallization trials and structure elucidation. Applicants must have demonstrated experience with methods used for protein purification, crystallization and structure determination. Experience with membrane proteins is not absolutely required. Salary will be commensurate with experience and is in addition to a generous fringe benefit package. For more information about the position or to apply, please email a current CV including a brief description of your research interests and accomplishments, and the names and addresses of two referees to Bert Poolman ([EMAIL PROTECTED]) or Dirk Slotboom ([EMAIL PROTECTED]). Please do not respond to me. Thank you. Ronnie Berntsson -- Ph.D. Student Department of Biochemistry Groningen Biomolecular Sciences and Biotechnology Institute Zernike Institute for Advanced Materials University of Groningen Nijenborgh 4, 9747 AG Groningen, The Netherlands telephone: +31 50 363 4195 telefax: +31 50 363 4165 e-mail: [EMAIL PROTECTED] homepage: http://www.rug.nl/gbb/research/researchgroups/enzymology/index
[ccp4bb] crystallization robot
Since everybody is showing off their kit (and we have been very well supported by Innovadyne): we at the LMB in Cambridge are extremely happy with our Innovadyne Screenmaker 96+8. It has been doing around 8000 plates per year for the past three years and it works well between 100 and 500 nl (using MRC crystallisation plates, of course). For more details: http://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/robot.html best, jan
Re: [ccp4bb] crystallization robot
What Jan says (in brackets) is true and very important. If you go to great lengths finding the best robot, you might as well use the best plates, and the MRC plates are the best by far that I've ever used. http://www.innovadyne.com/products_iplateSD2.html Andreas Jan Lowe wrote: Since everybody is showing off their kit (and we have been very well supported by Innovadyne): we at the LMB in Cambridge are extremely happy with our Innovadyne Screenmaker 96+8. It has been doing around 8000 plates per year for the past three years and it works well between 100 and 500 nl (using MRC crystallisation plates, of course). For more details: http://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/robot.html best, jan
Re: [ccp4bb] x ray data sets for teaching
The JCSG (www.jcsg.org) have absolutely buckets of high quality diffraction data which I imagine would be excellent for this task. These have the advantage of often being MAD data which will give a pretty decent map with a relatively straightforward shelx / solve script. Cheers, Graeme -Original Message- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Jeremiah Wagner Sent: 11 January 2008 14:58 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] x ray data sets for teaching Hello Everyone, I am going to be teaching a class this spring and protein structure and function. I would like to have my students see some diffraction data and integrate images with mosflm. Are there any free or available data sets (possibly lysozyme) out there that can be used? Thanks, Jeremiah Wagner --- Jeremiah Wagner Visiting Assistant Professor of Biology Beloit College 700 College Street phone: 608-363-2743 Beloit, WI 53511 FAX: 608-363-2052 --- Jeremiah Wagner Visiting Assistant Professor of Biology Beloit College 700 College Street phone: 608-363-2743 Beloit, WI 53511 FAX: 608-363-2052
[ccp4bb] Coot for Fedora Core 8?
hi, The subject tells it all. Is there somewhere a version of Coot that is suitable for Fedora Core 8? Thanks in advance, Fred.
Re: [ccp4bb] x ray data sets for teaching
Hi Jeremiah We (the Mosflm developers) use a mercury derivative HypF dataset collected on a Mar IP - there are things wrong with the crystal and the data collection, but it processes nicely (you can identify the problems easily), and you can solve the structure from the data; it's available at http://www.ccp4.ac.uk/autostruct/testdata/ - look for HypF, about half-way down the page. There are tutorials that go through the data with both the traditional X11 GUI and the new TclTk-based iMosflm on the Mosflm website. On 11 Jan 2008, at 14:58, Jeremiah Wagner wrote: Hello Everyone, I am going to be teaching a class this spring and protein structure and function. I would like to have my students see some diffraction data and integrate images with mosflm. Are there any free or available data sets (possibly lysozyme) out there that can be used? Thanks, Jeremiah Wagner --- Jeremiah Wagner Visiting Assistant Professor of Biology Beloit College 700 College Street phone: 608-363-2743 Beloit, WI 53511 FAX: 608-363-2052 --- Jeremiah Wagner Visiting Assistant Professor of Biology Beloit College 700 College Street phone: 608-363-2743 Beloit, WI 53511 FAX: 608-363-2052 Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, MRC Centre, Hills Road, Cambridge, CB2 2QH
[ccp4bb] x ray data sets for teaching
Hello Everyone, I am going to be teaching a class this spring and protein structure and function. I would like to have my students see some diffraction data and integrate images with mosflm. Are there any free or available data sets (possibly lysozyme) out there that can be used? Thanks, Jeremiah Wagner --- Jeremiah Wagner Visiting Assistant Professor of Biology Beloit College 700 College Street phone: 608-363-2743 Beloit, WI 53511 FAX: 608-363-2052 --- Jeremiah Wagner Visiting Assistant Professor of Biology Beloit College 700 College Street phone: 608-363-2743 Beloit, WI 53511 FAX: 608-363-2052
[ccp4bb] pirate and R-free
running pirate 0.4.9 w/ gui 1.4.4.2 ; the documentation suggests that R-free can be left unassigned - yet, pirate fails, claiming : colin-wrk-free /*/*/[Unassigned] CCP4MTZfile: import_hkl_data - Missing column, crystal or dataset: /*/*/Unassigned ... so i really need R-free? -bryan
Re: [ccp4bb] crystallisation robot
I sent this personal reply on wednesday to Madhavi Nalam's original question. In the light of recent comments I'm posting it to the BB to support TTP labtech and Mosquito! Some of the points have been made by others now, excuse the repeats! We at YSBL have been very happy with our mosquito. We use it to setup sitting drops with the MRC Wilden plate, this can be for screening or optimisation (not always necessary to go to 24-well format). You can also setup hanging drops, this can work better if detergents are involved. A new lid has just been released from TTP that costs £2.50 and takes 3 drops per well but we havn't tried this yet. You will need another robot (or multichannel pipette) for transfering the screen solutions.We use the hydra from alpha-helix ltd with flexible needles so hard to break. The phoenix robot does both jobs and seems popular. The Douglas robot has been mentioned, the Cartesian is the other main robot in use but I don't have any personal experience of it, I've heard it is quite high maintenance. Good luck with your purchase! Shirley Roberts begin:vcard fn:Shirley M. Roberts n:Roberts;Shirley M. org:York Structural Biology Laboratory adr:University of York;;Chemistry Department;York;;YO10 5YW;UK email;internet:[EMAIL PROTECTED] tel;work:01904 328268 tel;fax:01904 328266 url:http://www.ysbl.york.ac.uk/people/5.htm version:2.1 end:vcard
Re: [ccp4bb] Sulfate ion on 2-fold axis - Garib?
Best way of refining SO4 two-fold axis is to put al occupancies equal to 0.5 and refine like that. You may need to use newer version to deal better with atoms in special position. Have a look: www.ysbl.york.ac.uk/YSBLprograms/index.jsp You can go from this site to latest refmac site. Any further problems with this refinement please let me know. Garib P.S. You can put restraints between symmetry related atoms (you do not need them in this case) using keywords. There are keywords to deal with them. You can find them the same site as the latest refmac. On 11 Jan 2008, at 19:58, Jie Liu wrote: I've played around for a while, also read the manual and googled the internet, but just couldn't find a way to set up the restraints to the symmetry related atoms in Refmac5. Could anyone please kindly advice me how to do that? Thank you!!! Jie Eleanor Dodson wrote: It needs Garib to answer this! Eleanor Charlie Bond wrote: Eleanor Dodson wrote: It should be more or less equivalent, but better I think to put 1S at 0.5 occ and 2O at occ = 1 At least in REFMAC the restraints to the symmetry atoms should be set up But if you do that, some of the bond angles will be unrestrained, won't they?: O1-S-O2 will be OK, but O1-S-O1' and O2-S-O2' will not. Or is refmac cleverer than I think? Cheers, Charlie
Re: [ccp4bb] Sulfate ion on 2-fold axis - Garib?
I've played around for a while, also read the manual and googled the internet, but just couldn't find a way to set up the restraints to the symmetry related atoms in Refmac5. Could anyone please kindly advice me how to do that? Thank you!!! Jie Eleanor Dodson wrote: It needs Garib to answer this! Eleanor Charlie Bond wrote: Eleanor Dodson wrote: It should be more or less equivalent, but better I think to put 1S at 0.5 occ and 2O at occ = 1 At least in REFMAC the restraints to the symmetry atoms should be set up But if you do that, some of the bond angles will be unrestrained, won't they?: O1-S-O2 will be OK, but O1-S-O1' and O2-S-O2' will not. Or is refmac cleverer than I think? Cheers, Charlie
[ccp4bb] Speakers for APC/VD conference
Dear All: Here is an opportunity for junior (and also not so junior) fellows to speak at an interesting meeting: I had some oral presenters cancel the last minute for various personal reasons, and I have a few slots open for oral presentations at: - ADVANCES IN PROTEIN CRYSTALLOGRAPHY AND VIRTUAL DISCOVERY Conference and Exhibition 23-24 January 2008 Wyndham Hotel, Palm Springs, California, USA - http://www.ProtCrystConf.com http://www.VirtualDiscovery.net If you have something exciting as far as new techniques - experimentally as well as in computational crystallography or particularly virtual drug discovery/screening etc - are concerned, this a nice opportunity to present amongst a number of selected invited speakers. Please contact Sara Gadberry ([EMAIL PROTECTED]) for assistance with arrangements and allowances. Particularly for local speakers (CA etc) she can provide travel and other perks. Please send a title and short abstract to me as well. Hope to see you in sunny Palm Springs, Best regards, BR - Bernhard Rupp 001 (925) 209-7429 +43 (676) 571-0536 [EMAIL PROTECTED] [EMAIL PROTECTED] http://www.ruppweb.org/ - The hard part about playing chicken is to know when to flinch -
Re: [ccp4bb] crystallisation robot
Thanks to all who replied to my query. Madhavi
Re: [ccp4bb] crystallisation robot
Dear all - Under the BIOXHIT home page, http://www.bioxhit.org you can navigate to Section 1 or HTP Crystallization, or simply follow the link below: http://icarus.embl-hamburg.de/bioxhit/bioXHITSection1.jsp It has quite a lot of data on what people use, available facilities, and testing of some popular robots. If any lab/facility wants to contribute data to that resource please get in contact with me. My best regards, Tassos On 11 Jan 2008, at 19:25, Shirley Roberts wrote: I sent this personal reply on wednesday to Madhavi Nalam's original question. In the light of recent comments I'm posting it to the BB to support TTP labtech and Mosquito! Some of the points have been made by others now, excuse the repeats! We at YSBL have been very happy with our mosquito. We use it to setup sitting drops with the MRC Wilden plate, this can be for screening or optimisation (not always necessary to go to 24-well format). You can also setup hanging drops, this can work better if detergents are involved. A new lid has just been released from TTP that costs £2.50 and takes 3 drops per well but we havn't tried this yet. You will need another robot (or multichannel pipette) for transfering the screen solutions.We use the hydra from alpha-helix ltd with flexible needles so hard to break. The phoenix robot does both jobs and seems popular. The Douglas robot has been mentioned, the Cartesian is the other main robot in use but I don't have any personal experience of it, I've heard it is quite high maintenance. Good luck with your purchase! Shirley Robertsroberts.vcf
Re: [ccp4bb] Sulfate ion on 2-fold axis - Garib?
sorry for repeat. Link I gave in the previous mail should be: www.ysbl.york.ac.uk/YSBLPrograms/index.jsp Garib On 11 Jan 2008, at 19:58, Jie Liu wrote: I've played around for a while, also read the manual and googled the internet, but just couldn't find a way to set up the restraints to the symmetry related atoms in Refmac5. Could anyone please kindly advice me how to do that? Thank you!!! Jie Eleanor Dodson wrote: It needs Garib to answer this! Eleanor Charlie Bond wrote: Eleanor Dodson wrote: It should be more or less equivalent, but better I think to put 1S at 0.5 occ and 2O at occ = 1 At least in REFMAC the restraints to the symmetry atoms should be set up But if you do that, some of the bond angles will be unrestrained, won't they?: O1-S-O2 will be OK, but O1-S-O1' and O2-S-O2' will not. Or is refmac cleverer than I think? Cheers, Charlie
Re: [ccp4bb] Multiple Signature Motifs in a single protein
This depends of course on your definition of signature motifs, taking this in a broad sense - many proteins that have multiple functional domains probably fit the bill, so you could search for really huge proteins. For example: FAS (human, not bacterial) - 2504 amino acids in a single chain (!) Eukaryotic type II topoisomerases GYRA/B LRP1-6 Membrane-spanning phosphatases ... Artem -Original Message- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of DIC Sent: Friday, January 11, 2008 4:22 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Multiple Signature Motifs in a single protein Dear sir, I'm in something of a fix as I need to compile a dataset of proteins to test my work. The dataset would need to consist of proteins that contain multiple signature motifs. I am having difficulty finding any with more than two signature motifs. I would be grateful if you can let me know of any proteins that have multiple (three or more) signature motifs. Thank you in advance. Yours sincerely, Bioinformatics Centre Supercomputer Education and Research Centre Indian Institute of Science Bangalore - 560 012 E-mail : [EMAIL PROTECTED] Tel : 080-22932469 Ext:28 -- This message has been scanned for viruses and dangerous content by MailScanner, and is believed to be clean. -- This message has been scanned for viruses and dangerous content by MailScanner, and is believed to be clean.