Hi everyone,
I received a lot of positive e-mails about my post on cryo
methods. One theme was, "Well, what are the papers I should read?"
I know of one such paper published in Methods. I have made the PDF of it
downloadable from our web site at http://www.rigaku.com/cryo/
Cheers,
Jim
Hi Donghui,
I just want to draw your attention to the following article. It sums up
suggestions which were already made by others for using concentrated
(lithium) salt solutions for cryoprotection:
Rubinson, K.; Ladner, J.; Tordova, M. & Gilliland, G. Cryosalts:
suppression of ice formation in mac
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Dear group members,
I'd like to bring following opening to your attention:
-
Bruker AXS designs and manufactures analytical X-ray systems for elemental
analysis, materials research and structural investigations. Our innovative
solutions enable a wide range of customers in heavy industry, che
Dear group members,
I'd like to bring following opening to your attention:
-
Bruker AXS designs and manufactures analytical X-ray systems for elemental
analysis, materials research and structural investigations. Our innovative
solutions enable a wide range of customers in heavy industry, che
There are many excellent review articles about cryocrystallography and
cryoprotectants. Do labs generally have these articles handy in a methods
folder? Do lab heads help their colleagues by making them read them?
Mineral oil is generally not a good oil to use because it changes volume
too m
Donghui,
We have several membrane proteins crystallized in salt-based
conditions, actually in high sodium citrate. Yes, LiCl or lithium
formate are good alternatives, but also try lithium citrate, as
citrate also depresses the freezing point quite well.
Good luck,
Michael
*
Hi Brenda,
Brenda Patterson wrote:
I am doing molecular replacement with a model of sequence identity 42%.
If you used a CCP4 automated MR tool to generate your model, then the
"[Post MR] Fill Partial Residues" can conveniently restore side-chains
that have been "Schwarzenbachered".
Have
Dear all
I am doing molecular replacement with a model of sequence identity 42%. Have
been trying numerous combinations of weighting in refmac of CCP4. However the
geometry of the model is not being maintained. I am then trying manual fitting
of the model in COOT. However when torsion restraints a
Hi Rosemary
SC has stopped because P is not in its list of atomic radii. This list
is located at
$CCP4/lib/data/sc_radii.lib
You will need to edit this file to add a value for P ( and possibly some
of the other atoms in your RNA fragment). The format is fairly
self-explanatory, with asterisks
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Central London
(Reference SB0308)
Cancer Research Technology (CRT) is a specialist oncology company, which
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Here is an example:
http://news.gmane.org/gmane.editors.textmate.general
Charles Ballard wrote:
> Dear Mark
>
> gmane has an obscure e-mail address function. We would use that.
>
> Charles
>
> On 26 Feb 2008, at 16:58, Mark J. van Raaij wrote:
>
>> Dear Charles,
>> it would perhaps be good to
Dear Frank,
Here you mentioned the saturated stock 3M is at room temperature or not.
Since at room temperature now I can make 2.5 M stock at most at about 22
degredd celsius.
Thank you very much for your good suggestion.
Donghui
On 2/27/08, Francesco Rao <[EMAIL PROTECTED]> wrote:
>
> Hi Dongh
Dear Mark
gmane has an obscure e-mail address function. We would use that.
Charles
On 26 Feb 2008, at 16:58, Mark J. van Raaij wrote:
Dear Charles,
it would perhaps be good to make sure email addresses are not
publicly visible on gmane, to avoid spamming.
greetings,
Mark J. van Raaij
Dpto
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