Dear All :
I am studying a mutant protein related with a disease. In the mutated
version of this protein there is an alpha helical segment that protrudes
into the solvent. This alpha helix is not present in the wild type
protein.
I was wondering if any of you know some papers in which the
Dear CCP4rs
This morning we encountered a problem with ccp4mg in windows. It does
not start and a window entitled error in startup script pops in with
several lines referring to the code. Uninstalling and reinstallation of
the program did not help. Has anyone else encountered this ?
Thanks to everyone for their replies- very helpful! Below is a summary:
SEC-CAT 22-ID and 22-BM at APS will do both mail-in and remote
The canadian light source http://www.lightsource.ca/ has at least mail in
protein crystallography.
Remote access data collection is available on all the ESRF
May be old fashioned, but what about cut with ClaI, fill in,
and then you have a blunt end to work with.
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Artem Evdokimov
Sent: Sunday, July 20, 2008 12:02 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re:
Or do the new school cloning, SLIC (Sequence Ligation Independent
Cloning) and subclone into any position in a vector.
This method uses a PCR product and vector, the PCR primers have 20-40bp
overlap with a region in the vector. Mix cut and purified vector with PCR
product. Digest with T4
Dear all,
in a recent synchrotron trip we had a problem with our crystals moving
after mounting them onto the goniometer, in some cases they moved out
of the beam and even out of the zoomed camera picture - it seemed the
pins, upon equilibrating to room temperature, extended. It happened
...Or do the slightly-less-new-but-not-quite-old school technique:
QuickChange. Find a vector with roughly the properties you want, and
just quickchange
mutagenize in NdeI and ClaI sites at the desired positions. Works like
a charm
for me, assuming the vector is 10Kb or so, and I typically
I've seen haunted crystals before - the culprit was indeed with the
mounting of the pins in their bases (I was re-using some pins and
apparently the adhesive had cracked or otherwise failed). Fortunately I
never leave home without a tube of Duco cement and was able to correct
the problem in
Another new school cloning reference:
Klock, H.E., Koesema, E.J., Knuth, M.W. Lesley, S.A. Combining the
polymerase
incomplete primer extension method for cloning and mutagenesis with
microscreening
to accelerate structural genomics efforts. Proteins (2008) 71, 982-994.
published online 14
...Or do the slightly-less-new-but-not-quite-old school technique:
QuickChange. Find a vector with roughly the properties you want, and just
quickchange
mutagenize in NdeI and ClaI sites at the desired positions. Works like a
charm
for me, assuming the vector is 10Kb or so, and I typically
This reminded me of a haunted beamline that removed crystals from the loop.
You'd loop the crystals up nicely, block the stream, transfer the crystal fast
to the goniometer head, unblock the stream then look in the microscope - no
crystal! After a few tries (and head scratching) the culprit was
Hi Mark,
Cryocooled loops stored in tubes inside dewars do flex a little when the loops
are put on the goniometer. This is natural, as in the dewar, the whole pin was
in liquid nitrogen, but on the goniometer, it is only the loop that is in the
cryo stream. Having said that, the degree of
I recently had exactly this problem only I caught the crystal frozen
in the act of being catapulted out of the loop. I was using a thicker-
than usual oil for cryoprotectant and kept seeing empty loops with
what looked like long clear hairs attached. Finally, one loop had a
graceful arc of
Hi All,
Beamline 3BM1 at the Australian Synchrotron http://www.synchrotron.org.au has
full remote access available for users. We use the same Blu-Ice gui as SSRL and
use the SAM robot.
When 3ID is available for general user program (2009) it will also have full
remote access capabilities.
Dear All,
I want to prepare 25 mM sodium acetate buffer pH 4.5. can anyone give the
exact composition of how to prepare it. we prepare it using sodium acetate
and acetic acid combination. i am not able to arrive at the calculatation
correctly, so if anyone can explain me with the above buffer
This is a job for the trusty Henderson-Hasselbach equation:
http://en.wikipedia.org/wiki/Henderson-Hasselbalch_equation
On Jul 21, 2008, at 8:12 PM, Meg wrote:
Dear All,
I want to prepare 25 mM sodium acetate buffer pH 4.5. can anyone
give the
exact composition of how to prepare it. we
Dear all --
On 22 Jul 2008, at 00:45, Tom Caradoc-Davies wrote:
Beamline 3BM1 at the Australian Synchrotron
I believe in Japan as well there is a remote control available, at
SPring8. It is called Mail-in system. You can probably find more
informations there:
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