This has been said already but just to emphasise my view.
1) Measured intensities should never be lost and the TRUNCATED output
should/ (and does by default) keep them.
2) TRUNCATE extracts Fs from Imeass in bins; it is far from perfect but
we need amplitude estimates for some purposes and
Proteopedia - because life is not 2D
Proteopedia is a new interactive web resource that presents 3D
Biomolecular structures in an intuitive way by linking descriptive
text to 3D images (see e.g., HIV-1 protease http://proteopedia.org/wiki/index.php/HIV-1_protease
).
I'm interested to hear responses on this as well. We have shipped back and
forth crystals a 100 times and never had a problem until our last trip to the
synchrotron. The person at fed ex knocked our shipper onto it's side in order
to verify the measurements. The fact that they did it with a big
Dear All:
I have obtained good crystals from 20% isopropanol but this is my first
time dealing with this agent.
Help in handling the crystals, dealing with evaporation etc. is highly
appreciated.
Thank you
Subbu
I always used to just fill the shipper w/ LN2, and declare it Dangerous
Goods. Never any trouble, save one time in Birmingham (AL), the FedEx
person at the office intentionally tipped shipper 90 degrees, even
though it was marked DG, Keep Upright, etc. !! Naturally, they got
scared by all the
Dear CCP4 community,
Sorry for the off-topic subject, but I would really appreciate some
suggestions and/or protocols relating to native gel electrophoresis of
basic proteins. I have used a general acidic PAGE protocol for my protein,
which has a PI of 9.5. Briefly, the protein was loaded onto a
You could try Coomassie Blue Native gel. It's a very neat technique and it
worked for me on a couple of occasions. In one unfortunate case, it
resulted in dissociation of a heterotetrameric complex, though.
Artem
Dear CCP4 community,
Sorry for the off-topic subject, but I would really
Hi! Vacuum oil can effectively stop evaporation completely if immediately
dispensed onto the drop after the cover slip is removed from the well.
2x-5x the drop volume dispensed directly onto the drop creates a drop
within a drop, where air is prevented from reaching the inner drop, but the
outer
Dear All,
Thanks for the response.
For those of you interested, the extra cost associated with shipping
'wet' is about 35 USD within the US, and about 50 to 100 dollar
internationally. Extra paperwork and labels are needed when shipping
wet. One of our users recommends shipping wet, especially
Ming,
On that note, here is a Blue Native PAGE protocol that reportedly
improves the separation of basic proteins. A friend and I have used it
for native protein separation, but not necessarily for basic proteins.
Word of warning: don't use histidine salts or Tris-HCl in the cathode
Hi Ming,
You can find a protocol for low pH native gel electrophoresis (as well
for high pH and blue native) in the following book:
Gel Electrophoresis of Proteins: A Practical Approach (Practical
Approach Series) by B. D. Hames.
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