Hi Ian,
I did not follow up our recent debate about the respective merits of
various "truncate" procedures, in particular the comparison of the
French & Wilson (1978) and Sivia & David (1994) methods. Since you
have extended the previous simulations, I feel that the discussion
should be c
On Saturday 04 October 2008 18:32:00 Engin Ozkan wrote:
> Hi everyone,
>
> I was in the middle of creating a "Table 1" for a finished structure and
> was puzzled by one number. It is the average B factor, especially in
> the case of TLS-refined structures. In this case, the average reported
>
Hi,
Sadly, that happens sometimes.
1) make sure you have some salt in the DNA stock too
2) try (NH4)2SO4 instead of NaCl (just don't add Ca++, and
remember the ionic strength of a given molarity will be higher)
3) if you have to, try more salt
3) try different ends on your oligos - sometimes th
Hi Simon,
If you output no merge original index from scalepack, and (I assume)
don't impose any symmetry on the data, so scale in P1, pointless will be
able to work with the output. You will need a relatively recent version
of pointless though, from
ftp://ftp.mrc-lmb.cam.ac.uk/pub/pre
Best,
Gra
just use the "no merge original index" command, which you can add
during scaling using a macro. Just type "no merge original index" in
the macro field during scaling.
Then you'll have to import unmerged data to an mtz file using combat,
if I remember correctly.
hth,
ciao
sebastiano
Monday, Octobe
Hi everyone,
I would like to us pointless in CCP4 to check the laue group of a crystal.
Since pointless needs unmerged data, my question is how to generate the
unmerged data for pointless from HKL2000 ?
Thanks,
Simon
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