Re: [ccp4bb] foam dewar usage ?

I often use styrofoam boxes from dry ice shipments. Works like a charm if the box is quite new (i.e. not reused too often) -Original Message- From: "Smith, Clyde" <[EMAIL PROTECTED]> Subj: Re: [ccp4bb] foam dewar usage ? Date: Thu Oct 9, 2008 8:26 Size: 2K To: CCP4BB@JISCMAIL.AC.

Re: [ccp4bb] foam dewar usage ?

Maybe we're not talking about the same kind of container? We never had any kind of particles** in ours and with the use of a lid the ice does not appear for quite some time. We did not see 'vigorous boiling' either. If one leaves LN2 in the open it would eventually ice up regardless of the nature o

Re: [ccp4bb] Potential N-terminal cyclization

If you're 100% sure that this is only one atom then amination comes to mind. I have no clue what conditions would favor such reactivity but it is possible that the formyl group on the Met was aminated with the cyclic N of the histidine, resulting in either a substituted bis-amine (requires reductio

[ccp4bb] Potential N-terminal cyclization

Hi All, I am looking for some input regarding an unusual feature in one of our structures. Maybe someone has come across something similar or has some thoughts about it... We have a 1.9 A crystal structure with well-defined density in the N-terminal region. The side chain of His3 is oriented

Re: [ccp4bb] foam dewar usage ?

A side note about ice formation... This might have already been mentioned, but I have personally used a shower cap over cryo-bowls and it seems to do a reasonable job - ice seems to form quicker on the shower cap than in the bowl. Covering the bowl with an old Styrofoam lid from a spare shippi

Re: [ccp4bb] foam dewar usage ?

Just wanted to clarify the history of the foam Dewars for posterity: - they were developed at the ALS when Gerry McDermott was head of the BCSB group here, and Jon Spear was a member of the group (Gerry was his supervisor at that time) - after we had several glass Dewars implode, Gerry asked Jo

Re: [ccp4bb] foam dewar usage ?

For crystal freezing and such we have largely replaced glass and stainless Dewars with foam ones here at the CAMD PX beamline.You can get them directly from www.spearlab.com for USD 100.I agree that the lid supplied with the foam Dewar is not very good but you can easily make a

Re: [ccp4bb] foam dewar usage ?

Hi Carsten, since you don't use them anymore I would volunteer to have them shipped to me for free :-) I might cough up the shipping fee. We have both in the lab, for certain purposes I prefer the stainless steel over the foam dewars e.g. freezing protein as 20 µl drops then picking them

Re: [ccp4bb] foam dewar usage ?

> If they really do cost $160, your best bet might be to email your friendly > local synchrotron group and ask if they will make you something at a fraction > of the price ... Or just pay up. 160 USD is not a lot when compared to other consumables likes chemicals or crystal screens. The dewars l

Re: [ccp4bb] Foam Dewars Part II

Hi Alun, The material we use is cross linked polyethylene/EVA foam. We use the 4 PCF (pounds per cubic foot) variety it has tradenames of youngboard, artilon and epilon. (see http://smb.slac.stanford.edu/facilities/hardware/cassette_kit/dewar_schematics.pdf and http://smb.slac.stanford.edu

Re: [ccp4bb] foam dewar usage ?

I concur with Thomas' comments ... it all depends upon their intended use. We have used it extensively here at SSRL and have shaped three different large (4+ litre capacity) dewars for crystal mounting into the SSRL puck and for loading and unloading Uni-pucks in an adapter cassette for use wit

Re: [ccp4bb] foam dewar usage ?

A good cover -- judiciously used -- goes a very long way in reducing ice in dewars whether the dewar is made of foam, glass or stainless steel. If you keep your dewar covered with a lid that extends on the outside of the dewar down a few centimeters below the dewar edge, you will have minimal

Re: [ccp4bb] foam dewar usage ?

Funny indeed. Our experience has been as well that icing of the foam dewars seems to be reduced compared to the "classical" ones. Bert -Original Message- From: CCP4 bulletin board on behalf of Schubert, Carsten [PRDUS] Sent: Thu 10/9/2008 9:15 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re

[ccp4bb] Unsubscribe from CCP4

Please delete the email id [EMAIL PROTECTED] from your mailing list and BB. Thanks Sadhana Pullalarevu

[ccp4bb] Foam Dewars Part II

Hi All, I think the foam from these dewars would make excellent flooring in LN2 working areas (where most other flooring cracks). I have seen Judo mats made out of a foam that looks similar. Can anyone tell me what this foam is? Alun. -- Alun R. Coker University College London Division of

Re: [ccp4bb] disulfide bond in coot

Klaus Futterer wrote: Does anyone know how to prevent Coot from establishing a disulfide bond between two adjacent Cys residues? I have reason to suspect that the bond is (at least partially) reduced. It is not possible directly to stop Coot drawing the bond given your atoms. However, you co

Re: [ccp4bb] foam dewar usage ?

The low-form foam dewar is really nice to work with. Fills easily without excessive boiloff, and holds level pretty well while working with crystal loops and vials. Best of all, not much ice will accumulate at the top of the container while working. Best of all, my undergraduates can't break it

Re: [ccp4bb] foam dewar usage ?

We've loved them from ages ago when I had our workshop make some. (They didn't like making them, though, so thanks to SPEARLAB for doing it instead!) In fact, spearlab made a green lid on our request which overhangs the edge; I think it'll now be standard, or something like that. He also m

Re: [ccp4bb] foam dewar usage ?

Hmmm, that's funny. Our experience was quite the opposite. The first time we used one of the darker small dewars we ended up with a lot of burr in the LN2. Those got picked up by the cryo and we ended up with lilac particles embedded inside the cryo. You can imagine the reaction when these parti

[ccp4bb] disulfide bond in coot

Does anyone know how to prevent Coot from establishing a disulfide bond between two adjacent Cys residues? I have reason to suspect that the bond is (at least partially) reduced. Thanks. Klaus - Klaus

Re: [ccp4bb] foam dewar usage ?

Yes, we use them all the time and they're great. Stuff does not slip on the bottom like it does in the glass Dewars and the formation of ice is greatly reduced. Plus, they're much easier to dry. Artem > Does anyone in the biocrystallogaphy community use foam dewars for > handly liquid nitrogen an

[ccp4bb] foam dewar

Jean-Baptiste-- We switched to these foam dewars this year, & everyone in our lab uses them now. After having one of the old style glass ones explode during a late night synchrotron adventure, these foam dewars are a welcome change! annie Annie Hassell Glaxo Smithkline 5 Moore Drive RTP,

[ccp4bb] Postdoctoral position available at The Institute of Cancer Research

The Institute of Cancer Research Section of Structural Biology / Section of Cancer Therapeutics Post-doctoral Training Fellow Structural elucidation of phosphatase cancer target (Chelsea, London & Sutton, Surrey) The Institute of Cancer Research is one of the world'

Re: [ccp4bb] foam dewar usage ?

James, for a LN container like this I don't think people would even consider putting these plastic things into the autoclave for pasteurization. I had used these at GM-CA [EMAIL PROTECTED] more than once and always wish to have one in the lab - less ice formation on the rim, hence in the liquid in

Re: [ccp4bb] foam dewar usage ?

Jean-Baptiste, Since these were conceived and developed by Jon Spear when he was a member of my group (at the ALS at the time), I know a bit about these Dewars, their intended usage, and relative benefits. When Jon first made them, the beamline users and staff quickly realized the advantages,

Re: [ccp4bb] foam dewar usage ?

we have those 800ml dewars at our beamlines and I would not by them anymore. The green lid is not really insulating and the working area inside the cylinder is too small. In order to use it for sample manipulations, one has to fill it completely with LN2, which will cause uncontrolled LN2 spill

Re: [ccp4bb] foam dewar usage ?

They work fine and they don't break if you drop them. Bear in mind that they aren't resistant to a lot of chemicals and that you can't autoclave them. Also, the $160 price tag suggests a lucrative market niche for someone who has rudimentary skill with a carpet knife. On Oct 9, 2008, at 1:

[ccp4bb] foam dewar usage ?

Dear all, Does anyone in the biocrystallogaphy community use foam dewars for handly liquid nitrogen and freezing/manipulating frozen protein crystals ? We are interested in the following dewar package from Hampton Research : http://www.hamptonresearch.com/products/ProductDetails.aspx?cid=24&s

[ccp4bb] Epigenetic symposium, Nov 13/14

Dear All We would like to draw your attention to the forthcoming symposium: "Epigenetics Mechanisms in Health and Disease - From Biology to Medicine"     November 13th & 14th 2008 Said Business School, Oxford, UK This symposium will bring together clinical and preclinical scientists at