On Thursday 13 November 2008, Pavel Afonine wrote:
> Hi Ian,
>
> > All - I was just in a discussion about TLS and one thing that came out
> > that I hadn't been aware of is that for the Biso restraints Refmac
> > restrains the difference between the 'residual' Bs, i.e. with the TLS
> > contributio
Dear all,
Does anyone know of a good published reference that describes the pros
and cons of X-ray versus NMR structure determination. Something
specific to nucleic acids would even be better. I've noticed that
several papers describe NMR structures as "atomic resolution
structures" and I
GST is a dimer in any case,
the Bot NTa 1eh1 has a 37 residue N-terminal tag
we never saw it
BR
PS: does HTML mail cause any trouble re archive etc?
A nuisance for most basic communications purposes
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Beh
Hi Ian,
All - I was just in a discussion about TLS and one thing that came out
that I hadn't been aware of is that for the Biso restraints Refmac
restrains the difference between the 'residual' Bs, i.e. with the TLS
contributions subtracted, not the 'total' Bs. Now it seems to me that
this isn'
This has been reviewed, although a few years ago now. In most cases the
linker between the fusion tag and the protein of interest had to be
shortened and engineered. See
http://www.ncbi.nlm.nih.gov/pubmed/12824478?ordinalpos=2&itool=EntrezSystem2
.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultRe
Dear Users,
Through the Collaborative Crystallography Pilot Program (CC) at the
Advanced Light Source, scientists can send protein crystals to Berkeley
Center for Structural biology (BCSB) staff researchers for data
collection and analysis. The CC Pilot Program can provide an number of
benef
Dear All,
The link I send earlier was incomplete:
http://alsusweb.lbl.gov/4DCGI/WEB_GetForm/PXProposalEntry.shtml/Initialize
Excuses,
Peter
2008/11/13, Peter Zwart <[EMAIL PROTECTED]>:
> Dear All,
>
> The deadline for general user proposals for PX beamtime at the ALS is
> comming up soon! At
There are quite a few MBP fusions in the PDB. Just search using MBP sequence
and you will get (among others):
1A7L
1HSJ
1IUD
1MG1
1MH3
1NMU
1R6Z
And so on...
Artem
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of David
M Shechner
Sent: Thursday, Novemb
I have a more general question that's come up in discussion with former
colleagues: what's the largest tag that has been co-crystallized with the
target protein? I'm specifically wondering about MBP - we've encountered
several proteins that would express decently (and, apparently, correctly
folde
Many years ago, someone from UMass told me that he has crystallized many
proteins with GST-tag and believed that dimerization of GST helped
crystallization. I don't remember his name. --Chun
_
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
Nathaniel Echols
Sent: Thurs
On Thu, Nov 13, 2008 at 9:47 AM, iulek <[EMAIL PROTECTED]> wrote:
>
> But..., no one commented about c-myc tags. As I expected, there is no
> experience with this one yet - ?
>
I have a more general question that's come up in discussion with former
colleagues: what's the largest tag that has bee
Hi,
I didn't want to get into this because so many good comments and suggestions
have already been made - but I guess I will make a wordy and effusive
comment of my own :)
In my opinion, the question of how *specific* tags (His, c-Myc) influence
crystallization is somewhat misleading. The questio
Thanks for all who shared their experiences. I received some
personal and public suggestions/comments. So, time for a summary now.
My original question was about the influence of his-tag and
c-myc-tag on crystallization.
Concerning His-tags: some opinions differ, a brief summary of the po
Dear All,
The deadline for general user proposals for PX beamtime at the ALS is
comming up soon! At the BCSB, we have 5 beamlines for which you can
apply for beamtime. At the Berkeley Center for Structural Biology
(BCSB) we have recently completed a number of upgrades:
Sector 8: Recent improveme
Dear William,Paul(coot) and friends,
Everything seems fine, but when i did fink update-all, i encountered
following error,
one more thing, this fink is precompiled , i got for me from William scott,
and i still dont understand what options to choose
and how to install :
regarding dbus error,
(e
Dear all,
When we released iMosflm 1.0.0 on 17th October 2008, I accidentally uploaded
a zip archive with an old version of the imosflm source code for Windows. If
you downloaded the iMosflm for Windows zip archive between 17 October and 13
November 2008, you are running iMosflm 1.0.0 gc (gold can
Call for applications to the Membrane Protein Lab at Diamond
Applications are now invited for proposals to use the Membrane
Protein Laboratory (MPL) at the Diamond Light Source Ltd.
Closing date for applications: 4th December, 2008.
MPL website: http://www.diamond.ac.uk/Science/MPL
The MP
Dear all,
You are cordially invited to the
*Workshop "Neutrons and X-rays meet biology" *
25-27 February, 2009
Helmholtz Zentrum Berlin für Materialien und Energie
Deadline for abstract submission: 31 December, 2008
Registration fees: 140 euros (including, coffee breaks, meals, workshop
dinner,
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