Registration is now open for the EMBO practical course on Electron
Tomography in Life Science,
1-7 June, 2009, Leiden, The Netherlands
See http://cwp.embo.org/pc09-08/index.html for all course details
Application deadline is March 1st 2009.
where to start? 1st what is the resolution and Rfree of your model? Have
you cut the model down to 1 molecule in the ASU, and what does Matthew's
predict for your crystals as far as number in ASU? If all of those
answers are reasonable, floppy regions can often cause failure in MR. I
would
I had the same problem with an even higher %identity once (80%), eventually
resorted to SIRAS phasing with a mercury compound, and I found a case of
domain swapping creating a novel dimer interface.
Flip
