Re: [ccp4bb] Radiation damage with crystals containing metal centers (TaBr people chime in?)

2010-10-05 Thread J. Preben Morth
For this particular project, we mounted two native crystals from the crystal drop before adding the Ta6Br12(2+). The Ta6Br12(2+) was added as powder and left o/n. Since various amounts of Ta6Br12(2+) was added this way we never knew what the concentration was, many crystals were ruined his

Re: [ccp4bb] R factor R free struck

2010-10-05 Thread Roger Rowlett
As always, look at the crystal packing of the MR solutions. That is a quick way to reject incorrect space group solutions. I never realized Phaser had the issue you described with the SGALTERNATIVE setting, but to be honest, I've always run it in "manual" mode, i.e., I set up separate jobs in

[ccp4bb] met auxotroph S.cerevisiae strain?

2010-10-05 Thread tommi kajander
Hello all, Would anyone happen to have a met auxotroph S. cerevisaea strain at hand that you could send some of to us for testing Semet labelling? Thanks very much! Regards, Tommi Tommi Kajander, Ph.D., Macromolecular X-ray Crystallography Research Program in Structural Biology and

Re: [ccp4bb] met auxotroph S.cerevisiae strain?

2010-10-05 Thread Martin Hallberg
Hi Tommi, In S. cerevisiae you need a sam1(-) sam2(-) mutant. You can contact the authors of the paper in this link to get them: http://www.pnas.org/content/104/16/6678.long Cheers, Martin On Oct 5, 2010, at 3:47 PM, tommi kajander wrote: Hello all, Would anyone happen to have a met

[ccp4bb] stereo (again - sigh)

2010-10-05 Thread David Roberts
I hope for everybody this is the last time with this. I know it has been asked (even partially by me), and I know people talk about it often - but I am really trying to solve this problem once and for all, and am having a very hard time. Past and present set-up for this: Started out with

Re: [ccp4bb] stereo (again - sigh)

2010-10-05 Thread Ben Eisenbraun
Hello Dave, I would like to replace the CRT's with some sort of LCD thing. You have two options for stereo on linux. The Zalman passive stereo route, which you tried, or the active stereo route, which means an Nvidia 3d Vision emitter/glasses combo and a 120 Hz LCD. The glasses/emitter kit is

Re: [ccp4bb] stereo (again - sigh)

2010-10-05 Thread Harry Powell
Hi there is at least one other option - we have a Planar Systems 3D setup (uses two flat screen monitors mounted at ~right angles with a semi-silvered screen between them) - you use polarised lens glasses to view the stereo. It works extremely well, but is pricey. If you have a good workshop

Re: [ccp4bb] stereo (again - sigh)

2010-10-05 Thread Alexander . Schiffer
Dear all, We tested both the planar system you mentioned and the Viewsonic 120Hz TFTs and purchased the 120Hz TFT solution not only because of the price but we found the stereo quality to be equal and the usability much better for the 120Hz TFTs. With the planar system you have to move away the

Re: [ccp4bb] stereo (again - sigh)

2010-10-05 Thread David Roberts
Thank you all for the quick replies. I did leave out some specs, but I really wanted to know what others have found. Sadly, it's no different than my findings. I am at a small undergrad only university - low on resources but big on ideas for teaching students. This is not research based,