Dear Everybody,
it would have been nice to know how to overcome the following error of Refmac5
refinement (both for Rigidbody and Restrainst)
C:/Ccp4Temp/CCP4/DepositFiles/unknown/unknown061110:19:35:08.refmac has no
associated file name
$$
Open failed: Unit: 9, File:
Hi Laurie,
nuclear protein, cysteines, my thumbs prickle it might be a zinc-binding
protein.
Although 12 Cys per 257 aa is not yet that much. If you have always DTT
present oxidation should be no problem.
Reconstitution of zinc sites can be very tricky and is not
straightforward. Also E.coli
