Hi all,
Thank you all how have replied for their kind suggestions and diagnosis of
collected data.
sincerely
Debajyoti
On Sat, 26 Mar 2011 04:44:42 +0530 wrote
>
>
Hi all,
>
>
I have collected one iodine soaked data in our home source, and processing the
data using HKL2000. Exposure tim
If you want to use absorption spectroscopy, it depends on whether or not the
heavy atom under investigation have significant absorption at the X-ray
wavelength you have at home lab. If that is the case, you should be able to see
the absorption and tell whether or not the heavy atom has been inco
Collect all your diffraction data in your home lab, solve the phase problem,
fit the map, refine, deposit coordinates in the PDB.
_
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Careina Edgooms
Sent: Friday, March 25, 2011 2:40 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject:
Try taking the detergents out of your solublization buffer (that is if you know
it's not a membrane protein) and only extract the buffer soluble portion. If
you have a tag try switching the tag to a different terminus.
Dr. Paul Kraft
Structural Biologist
cell 586-596-2770
email: haresea...@yahoo
Hi all,
As part of its recent winter update, the Protein Data Bank in Europe (PDBe;
http://pdbe.org) introduced Quips - "QUite Interesting PDB Structures". Quips
are short stories about one or more interesting or topical structures, coupled
with an interactive viewer and often a tutorial that
The most likely explanation is that you have a cracked crystal or your
crystal has split from radiation damage around the frame 70.
If you had a cracked crystal from the start, the spot overlap between
crystals would change when the sample was rotated. In such cases, it may
happen that the program
Hi all,
I have collected one iodine soaked data in our home source, and processing the
data using HKL2000. Exposure time per frame is 5min/1 degree.
While processing I have noticed that the Chisq values, cell parameters and
rotation change Vs frame are deviating like anything. Please find the p
We use the Millipore Spiral-wound TFF1 and TFF2 cartridges for
concentrating 10-20L of insect cell media. They work great, don't
cost all that much money, and you don't need the expensive holder they
sell. You can use a ring stand and clamp the tubing directly to the
cartridge. Our cartridges ar
*Living deep: Structural basis of piezophilic adaptation*
ELMA group. Institut de Biologie Structurale J.-P. Ebel -- Grenoble --
France
http://www.ibs.fr/groupes/groupe-extremophiles-et-grands/
We are recruiting a post-doctoral scientist interested in life
adaptation to extreme conditions. The
On Mar 25, 2011, at 08:39 , Careina Edgooms wrote:
> Dear ccp4 users
>
> I would like to know, is there a way to check that heavy metal has bound to
> crystals before I take it to synchrotron which is far away?
>
> thanks
> Careina
Here's a useful reference:
Boggon TJ, Shapiro L
a quick google search turned up:
http://people.mbi.ucla.edu/sawaya/tutorials/Phasing/gel.pdf
Acta Cryst. (2000). D56, 161-168[ doi:10.1107/S0907444999015188 ]
Mass-spectrometry assisted heavy-atom derivative screening of human Fc<>RIII crystals
P. D. Sun and C. H. Hammer
Mark J van Raaij
yes u can run Native page and on that if u will see shift then crystals
containing heavy atom
regards
On Fri, Mar 25, 2011 at 1:09 PM, Careina Edgooms
wrote:
> Dear ccp4 users
>
> I would like to know, is there a way to check that heavy metal has bound to
> crystals before I take it to synchrotr
Dear ccp4 users
I would like to know, is there a way to check that heavy metal has bound to
crystals before I take it to synchrotron which is far away?
thanks
Careina
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