Did you run your crystals on a gel to ensure and verify that what you think you
crystallized is at least the right molecular weight ? Maybe the N-terminal part
is just chopped off and you have indeed crystallized just the 23 kDa fragment ?
Try running Xtriage or pointless over your data to
Nice find! ;-)
Just in case anyone want to see it IRL
http://www.youtube.com/watch?v=4sYSyuuLk5ghd=1t=38s
Regards,
Folmer
2011/7/16 Artem Evdokimov artem.evdoki...@gmail.com:
Fellow structural biologists,
I just caught a brief glimpse of a crystal structure (looks like an Fv
complex or
Hi Artem,
Thank for that nice example of a protein structure used to pimp a movie. Ribbon
representations are always the scariest.
Cheers,
Robbie
Date: Sat, 16 Jul 2011 10:57:21 -0500
From: artem.evdoki...@gmail.com
Subject: [ccp4bb] unusual sighting of a crystal structure
To:
The R and Rfree are low enough so your space group is correct. I don't see a
resolution in the log file. If it is 3 A dataset, 28% is not bad for a start
at all. The N terminal region is probably completely disordered if it is not
degraded.
Nian
On Sat, Jul 16, 2011 at 3:47 AM, Appu kumar
one molecule of 37 kDa with solvent content 17%
I highly doubt it. It's theoretically possible, but with extremely low
solvent content like this the crystals should be tightly packed, with
all the consequences of tight packing such as higher resolution
diffraction and most of the bits accounted