[ccp4bb] chirality problem

2012-01-05 Thread Afshan Begum
Dear Users, I am facing difficulties to validate my structure according to PDB server. I have solved my structure and now want to submit in PDB but during validation process i have  some chirality problem specially   VAL and LEU amino acids there are total 18 amino acids which deviated from

Re: [ccp4bb] chirality problem

2012-01-05 Thread Robbie Joosten
Hi Afshan, Just swap the (names of) the CD and CG atoms, no need for refinement. The CCP4 dictionary allows both chiralities for LEU and VAL, so Refmac won't detect the problem. The problem is still very real to many programs so it should be fixed. Cheers, Robbie Joosten Date: Thu, 5 Jan 2012

Re: [ccp4bb] From non-twinned to twinned?

2012-01-05 Thread Zhiyi Wei
Thanks. Your suggestion remind me that I forgot to mention one more information. Actually, I did try to shot different regions of crystal to reduce radiation damage. Say, the sca1 set used one region and the sca2 set used another. Is it possible that this two regions has different crystal domain

Re: [ccp4bb] live streaming of ccp4 study weekend

2012-01-05 Thread Charles Allerston
How is everyone finding the stream? It was extremely flaky this morning here, cutting out every 15-20 seconds. The afternoon session doesn't seem to be faring any different at this end, sadly. Just wondering if it is a problem at our end or theirs. cheers charlie -Original

Re: [ccp4bb] live streaming of ccp4 study weekend

2012-01-05 Thread David Mueller
I can't get it to work on Firefox, Safari, or Chrome using a Mac with OS 10.6.8. David Mueller On 1/5/12 8:31 AM, Charles Allerston charles.allers...@sgc.ox.ac.uk wrote: How is everyone finding the stream? It was extremely flaky this morning here, cutting out every 15-20 seconds. The

Re: [ccp4bb] live streaming of ccp4 study weekend

2012-01-05 Thread Edward Snell
Hi Charles, For both the morning (after I got up) and afternoon session slides have been fine - no video or audio though. A connection to the server could not be established :( Had to update my RealPlayer to a Nov 2011 release before seeing anything. Cheers, Eddie Edward Snell Ph.D.

Re: [ccp4bb] live streaming of ccp4 study weekend

2012-01-05 Thread Hazel
I also had the same problem, I could only get between 2-30secs at a time, whichever browser I used. I thought it might be my connection but maybe not if you're having the same problem. Will the videos be available for download afterwards? Hazel On Thu, 5 Jan 2012, Charles Allerston wrote:

Re: [ccp4bb] live streaming of ccp4 study weekend

2012-01-05 Thread Francis E Reyes
The video works fine if you load http://extrplay.dl.ac.uk/CCP4/20120105-2/rnh.ram as a network stream into VLC (www.videolan.org) . Trying to play it with real player just didn't work. And VLC doesn't require your admin password to spew garbage all over your system. You don't get the jpg

Re: [ccp4bb] live streaming of ccp4 study weekend

2012-01-05 Thread Andreas Förster
For what it's worth, I get it on Firefox and Safari using a Mac with OS 10.6.8. The sound is fine but the slides don't update automatically. I have to hit reload at every slide. Keeps me awake. Andreas On 05/01/2012 2:38, David Mueller wrote: I can't get it to work on Firefox, Safari,

Re: [ccp4bb] From non-twinned to twinned?

2012-01-05 Thread Ed Pozharski
On Thu, 2012-01-05 at 20:40 +0800, Zhiyi Wei wrote: Is it possible that this two regions has different crystal domain arrangement (one is normal and another is twinned)? Absolutely. This will, of course, vary for different crystal systems, but from what I have seen it appears that a