Dear Debajyoti,
It is likely that you have crystallized a contaminant in your preparation. You
can put the unit cell into BALBES which will tell you if the PDB has seen it
before. Another thing is to submit your polyalanine trace to DALI to see if the
fold is recognised.
James
--
Dr. James
Dear Michael,
the purpose of our paper (http://www.sciencemag.org/content/336/6084/1030) was
to develop and document methods that enable to check for yourself what the best
high resolution cutoff is - no rules of thumb are necessary!
However, repeated use of the paired refinement technique (3
hi all,
I got a protein with good condition without precipitating in the purification
process.And the protein is stalbe at 4oC, but when i try crystallization
screens, it precipating in all the kit(with PEG), in the kit with high
concentration of salt ,the situation was better(but still no
Hi,
The idea used by the Qiagen pre-screen might be relevant to your case:
http://www.qiagen.com/products/protein/crystallization/screeningproteincrystallizationconditions/prescreenassay.aspx
http://www.qiagen.com/literature/handbooks/literature.aspx?id=239
Zhijie
From: fengjuanlv12
Hi Fengjuan,
It has been proposed that for a specific protein there are hard precipitants
and soft precipitants. Hard ones precipitates the the protein effectively,
while soft ones may help stabilizing the protein. By playing with the two types
of precipitants, one may get better
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Hi folks,
Sorry for the cross post / off topic question. I have just noticed a
recurring/repeatable problem when using the get monomer option in Coot. E.g.
when getting ILE (and a few others) but not ala, Coot freezes on a windows 7
machine (using 64-bit thingies). I am using 0.6.2 and there
