Dear Dipankar,
To me, it looks like you have a (slight) problem with your goniometer/detektor
in that e.g. the rotation axis is not exactly horizontal/vertical/perpendicular
to the beam, or that the detector parameters (especially when using a
swing-out) were not completely correctly refined
Dear all,
Could anybody please tell me how to get the coordinate file and the cif
file for the trigonal bipyramidal (TBP) phosphorus (figure given below for
your convenience)? Prodrg server is not helping out in this issue. Thanks
in advance.
Regards,
Sudipta.
Sudipta Bhattacharyya
Senior
Dear Sudipta,
Use Phenix eLBOW. Give the input SMILES string and get the pdb and cif
restraint files as output. Also you can use
http://cactus.nci.nih.gov/translate/ to first get the pdb coordinate file from
the SMILES string and then run Phenix eLBOW to get the cif restraints.
Best regards,
EXCELLENT !!!
Dr Felix Frolow
Professor of Structural Biology and Biotechnology, Department of Molecular
Microbiology and Biotechnology
Tel Aviv University 69978, Israel
Acta Crystallographica F, co-editor
e-mail: mbfro...@post.tau.ac.il
Tel: ++972-3640-8723
Fax: ++972-3640-9407
Just a reminder that the closing date (30th September) for registering for this
BioSTRUCT-X funded meeting is fast approaching. The meeting is aimed at
synchrotron users wishing to get the most out of multi-circle goniometers /
pixel array detectors.
Workshop on Advanced Data Collection
On Sep 6, 2012, at 9:00 AM, eugene.krissi...@stfc.ac.uk wrote:
If this does not work for you for any reason, please (re-)read update manual
for details. If that does not help as well, please write to us.
Dear Eugene:
Will there be a way to get the corresponding changes to the source-code?
You may want to check that the distance, 2-theta and wavelength are correct.
Kris F. Tesh, Ph. D.
Department of Biology and Biochemistry
University of Houston
From: herman.schreu...@sanofi.com herman.schreu...@sanofi.com
To: CCP4BB@JISCMAIL.AC.UK
Sent: Tue,
Dear crystallography community,
I wanted to make the community aware of a new tenure-track faculty position
at Brown University in structural biology. Details about the position are
provided below.
*Structural Biology Faculty Position, Assistant Professor*
The Department of Molecular Biology,
Hello,
I am developing an undergraduate biochemistry lab class and
would like to incorporate experiments with membrane proteins. Does
anyone have suggestions on membrane proteins that are relatively easy
to express, purify, and assay? Bonus points for crystallizable! At the
moment, my
Wouldn't bacteriorhodopsin be a good choice, especially since it's colored
and easy to express? Seems pretty expensive for a class, though, with all
the detergents involved...
JPK
On Tue, Sep 11, 2012 at 4:18 PM, Ho Leung Ng h...@hawaii.edu wrote:
Hello,
I am developing an
Two additional suggestions:
1. Photosynthetic Reaction Center from *R. sphaeroides* might be up your
alley. Similar to bacteriorhodopsin, no need for a GFP tag on this one as
it's colored. See the following publications:
Wallace et al. Monoolein Lipid phases as incorporation and inrichment
Hi,
Just for info if you were to use the LCP method (a course by itself), check
this about OmpF (membrane lysozyme):
http://www.sciencedirect.com/science/article/pii/S1047847712000834
bR protein sometimes takes weeks to give crystals and people prefer the
dark (depends on the conf state).. but
The multidrug efflux transporter AcrB could be suitable including
crystallization (both recombinant with His-tag and endogenous). Extracts,
solubilizes, purifies and crystallizes in DDM, so it is fairly inexpensive to
repeat in bulk compared to other detergents. Assays might be a bit
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