Re: [ccp4bb] anti-His6 Ab

2013-03-16 Thread Juha Vahokoski
http://www.mail-archive.com/ccp4bb@jiscmail.ac.uk/msg27397.html Since the discussion, we tried qiagen ab, and I was quite happy with it. I did not do extensive performance tests, but it was better than the previous ab. Regards, Juha On Friday, 15 March 2013, Elias Fernandez wrote: Dear All,

Re: [ccp4bb] Resolution and data/parameter ratio, which one is more important?

2013-03-16 Thread dusan turk
Dear Guangyu Zhu, if this is not a hypothetical case you can refine both structures in each crystal form separately using whatever software and compare them later. The structure can be refined also in both crystal forms simultaneously using the multi crystal NCS refinement as implemented in

Re: [ccp4bb] CCP4 Update 019

2013-03-16 Thread eugene . krissinel
Spasibo Andrey, Ne znayu poka kak na Linuxe i vse ostal'noe, no na Mac superpose ne proupdeitirovalas', matritsy ne pechataet. Poka ne znayu chto sluchilos' Eugene On 15 Mar 2013, at 23:51, Andrey Lebedev wrote: Dear CCP4 Users A CCP4 update has just been released, consisting of the

[ccp4bb] Fwd: [ccp4bb] CCP4 Update 019

2013-03-16 Thread eugene . krissinel
Apologies for everybody, the previous message was sent to BB by mistake, please disregard. Eugene Krissinel Begin forwarded message: Date: 16 March 2013 11:25:08 GMT To: andrey.lebe...@stfc.ac.ukmailto:andrey.lebe...@stfc.ac.uk Cc: CCP4BB@JISCMAIL.AC.UKmailto:CCP4BB@JISCMAIL.AC.UK Subject: Re:

Re: [ccp4bb] first use of synchrotron radiation in PX

2013-03-16 Thread James Holton
The first report of shooting a protein crystal at a synchrotron (I think) was in 1976: http://www.pnas.org/content/73/1/128.full.pdf that was rubredoxin The first PDB file that contains a SYNCHROTRON=Y entry is 1tld (trypsin), which was deposited in 1989:

Re: [ccp4bb] first use of synchrotron radiation in PX

2013-03-16 Thread Bosch, Juergen
Thank you James, you should write a History book about the modern x-ray times. Or better make one of those movies you are famous for. Jürgen On Mar 16, 2013, at 10:46 AM, James Holton wrote: The first report of shooting a protein crystal at a synchrotron (I think) was in 1976:

Re: [ccp4bb] Resolution and data/parameter ratio, which one is more important?

2013-03-16 Thread James Holton
Well, when it comes to observations/parameters, it is important to remember that not all observations are created equal. 10,000 observations with I/sigma = 1 are definitely not as desirable as 10,000 observations with I/sigma = 10. Not all parameters are created equal either. Yes, you may

Re: [ccp4bb] first use of synchrotron radiation in PX

2013-03-16 Thread Ethan Merritt
On Saturday, 16 March 2013, James Holton wrote: The first report of shooting a protein crystal at a synchrotron (I think) was in 1976: http://www.pnas.org/content/73/1/128.full.pdf that was rubredoxin The first PDB file that contains a SYNCHROTRON=Y entry is 1tld (trypsin), which was

Re: [ccp4bb] qtrview command line options

2013-03-16 Thread Andrey Lebedev
After ccp4 update No 19, Log–files can be opened with qtrview from the command line: logview name.log This also works for log-files generated with quick scale and quick symmetry from imosflm. If ccp4 database entries do not exist, input and output files will not be shown in the viewer