Nat,
What do "correct" B-factors look like? What refinement strategy did you
> use for them?
>
1) If I see strong positive density in the Fo-Fc map along the backbone of
two turns of an correctly placed alpha helix, therefore the B-factors are
too high in that region. The model after refinement
Engin Özkan wrote:
Also, why would we be using the batch-wise I/sigma's for determining
resolution cutoffs?
Agreed. For cutoff I guess it should be the final sigma going into the refinement program that matters, irrespective of
whether that was achieve by averaging more images or using a stro
Hi,
On Tue, Jul 23, 2013 at 10:11:04AM -0500, Engin Ozkan wrote:
> Isn't the reported Mean(I/sigI) in the reported Aimless table for
> the merged/averaged reflections (because that is what we are
> discussing about)?
Yes - according to
1.
http://ccp4wiki.org/~ccp4wiki/wiki/index.php?title=Symme
On 7/23/13 9:01 AM, Edward A. Berry wrote:
Here we need some clarification on which I/s(I) is meant - sigma(I)
for the
individual measurements, or sigma(I) from error propagation to the final
which as Engin notes will be lower.
Isn't the reported Mean(I/sigI) in the reported Aimless table fo
Dear All
We will be hosting the 4th MX User Workshop at Diamond as part of the
Synchrotron User Meeting, September 4th-5th, 2013. This year the workshop
programme will cover new beamline capabilities, data processing and techniques.
The meeting will also feature updates from the Membrane Protei
Engin Özkan wrote:
On 7/22/13 11:20 PM, Ethan Merritt wrote:
Which brings up the point that something seems to have
gone wrong in one of your processing runs.
Both runs claim mean (I/sigI) in the outer shell is 2.0,
but in one case this is for the 2.4A shell and in the other case
it's for the 2
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Hi
I'd agree with Kay here - since the edge of the detector is at ~2.8Å. It is
almost always worthwhile integrating to a higher resolution than you can see
spots on the images - for what I would call "normal" datasets, I would always
integrate to ~0.2Å higher (as a first estimate), then after e
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Hi Stefan,
you write
"The diffraction pattern looks great, the 3.4A reflections are visible by eye
and the edge of the detector is about 2.8A."
and for the 3.4A data Mean((I)/sd(I)) in the highest shell is 2.3 .
I'm tempted to ask: what prevents you from using higher resolution data to,
say
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