Dear all,
a CRUK-funded postdoctoral fellowship is available at the London Research
Institute. We are looking for either (i) a biochemist interested in
reconstituting multi-component protein complexes, (ii) a crystallographer
motivated to integrate X-ray and cryo-EM approaches, or (iii) a
Dear all,
there has been ample discussion on this bulletin board regarding the
benefits of making diffraction images available for published X-ray
structures.
I'm considering implementing a repository at Imperial College, but I
don't want to reinvent the wheel or create something out of
Baerbel,
Certainly, constraints of the crystal lattice affect everything. The
magnitude of the influence is what is in question here. Let's do the
numbers (this is admittedly simplistic but reflect general trends well).
Let's say you observe difference in occupancies for two identical sites
http://www.bbc.co.uk/news/science-environment-25020112
Frederick Sanger: Double Nobel Prize winner dies at 95
--
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All Things Serve the Beam
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Good day!
I am refining what appears to be O-mannosylated protein structure.
In my hands Coot (0.7.1) does not form the SER-MAN bond automatically.
I made a SER-MAN.cif with jLigand, which takes care of the glycosidic bond.
However, now the peptide bonds are not made to this custom residue
Dear Crystallographers,
I dialysed a 30 kDa protein
(Recombinant protein which was eluted by 20 mM Tris, 500 mM NaCl, 120 mM
imidazole) against water for overnight. But it gets precipitated after 12
hours. Can anybody give some suggestion to avoid
On 20 Nov 2013, at 21:56, Dhanasekaran Varudharasu wrote:
Dear Crystallographers,
I dialysed a 30 kDa protein
(Recombinant protein which was eluted by 20 mM Tris, 500 mM NaCl, 120 mM
imidazole) against water for overnight. But it gets precipitated
Dear CCP4 users
Two new updates for the CCP4 6.4.0 series have just been released.
NB. If your CCP4 installation was damaged by previously released update No 2,
please read NOTES at the end of this e-mail.
Update 6.4.0-003:
This is a technical update to repair the update mechanism. You will
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Hash: SHA1
Hello Dhana,
use a buffer at least, and some salt - if I remember correctly, gel
filtration resins require 150mM salt concentration for proper
separation (might have been 50mM). Even 150mM may be too little to
keep your protein soluble.
Best,
Tim
Right on target. Many proteins require some ionic strength in the
solution to maintain solubility and prevent protein aggregation. Usually
NaCl is used for this purpose. You can also use glycerol to enhance
solubility, but this may interfere with crystallization if that's the
next step. NaCl
Hi Dmitry,
I think the best way is not to make a new monomer. MAN-SER and MAN-THR
linkages do exist in the ccp4 monomer lib. If you simply build a mannose
with its O1 removed and put the C1 ~1.4 Angstrom to the OG of the serine I
think refmac should be able to detect the linkage. When this
Dear All,
I am running autosharp with a single wavelength data soked with Ta6Br12.
This data collected at the wavelength of 1.254A. I told the autosharp the
f' -20 and f''10.5. The autosharp result said these values are not correct?
How can we get the f' and f'' of this cluster? Thank you.
Lisa
Dear Lisa,
if you have proper anomalous data I rather recommend using the Phaser
SAD pipeline and specify cluster search.
Worked instantly for me.
Good luck!
-
Dr. Matthias Zebisch
Division of Structural Biology,
Wellcome Trust Centre for Human
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