[ccp4bb] PhD positions in NMR spectroscopy/structural biology of photosynthetic membrane proteins at Leiden University, The Netherlands

2014-01-14 Thread Anjali Pandit
Currently, PhD positions are available at Leiden University, Leiden Institute of Chemistry, within the project in shape for photoregulation: molecular plasticity in photosynthesis financed by a VIDI grant of the Netherlands Organization of Scientific Research. *Job description* The

[ccp4bb] ACA 2014 meeting--Session on engaging students in protein crystallography

2014-01-14 Thread Roger Rowlett
I am co-organizing (with Kraig Wheeler) a session at the 2014 American Crystallographic Association Meeting in Albuquerque, NM concerned with engaging undergraduate students in protein crystallography. I would like to encourage anyone who has involved undergraduate students in protein

[ccp4bb] refining nucleic acids with Coot

2014-01-14 Thread Almudena Ponce Salvatierra
Dear all, I am trying to refine an oligonucleotide chain in Coot and I get the following message when I try: Fail to match (to the dictionary) the following model atom names: G O5* C5* O4* etc That would cause exploding atoms so the refinement did not start Does anyone know how to solve

Re: [ccp4bb] refining nucleic acids with Coot

2014-01-14 Thread Paul Emsley
On 14/01/14 15:51, Almudena Ponce Salvatierra wrote: Dear all, I am trying to refine an oligonucleotide chain in Coot and I get the following message when I try: Fail to match (to the dictionary) the following model atom names: G O5* C5* O4* etc That would cause exploding atoms so the

Re: [ccp4bb] refining nucleic acids with Coot

2014-01-14 Thread Almudena Ponce Salvatierra
Thank you all very much for your very helpful advices! I managed to solve my problem! :-) Best, Almudena. 2014/1/14 Ashley Pike ashley.p...@sgc.ox.ac.uk Hi Almudena – I came across this issue recently as well with an old pdb file. As Paul says you need to convert from v2.3 to v3.2 pdb

Re: [ccp4bb] autosharp error message

2014-01-14 Thread Clemens Vonrhein
Dear Almudena, On Tue, Jan 14, 2014 at 07:34:21PM +0100, Almudena Ponce Salvatierra wrote: Dear all, I find this error message while running autosharp: No column FP of type F found in file /usr/local/sharp/users/sharp/None.sharp/datafiles/xxx.mtz! Does anybody know how to

Re: [ccp4bb] ACA 2014 meeting--Session on engaging students in protein crystallography

2014-01-14 Thread Jim Pflugrath
Hi Roger, We have a simple lysozyme project where we collect diffraction images in less than 30 seconds total exposure time and solve the structure by SAD phasing. I've thought that this might make an ideal lab practical: grow crystals one week, everyone collects the data on their own crystal

[ccp4bb] Circular Dichoism 280nm range signal

2014-01-14 Thread JinSoo.Bae
Dear all, Sorry for the off topic. We are comparing the high order structure between Fc engineered mAbs using CD methods. There are some different (noise ?) signal in the 280nm range each antibodies. Other region is almost close match with respect of peak shape and theta value. Is there any