Dear Jacob,
There are a lot of potential problems with ion validation, that make obtaining
a reliable answer difficult. If you want to datamine ion validation results,
you can use the ready-made WHAT_CHECK files in the PDBREPORT databank for
original PDB files or in the PDB_REDO databank for th
Thanks! we're gonna test one of the lg's from b&h, one of our zalman's just
died today.
On Thu, Mar 6, 2014 at 6:06 PM, Shaun Lott wrote:
> Thanks to everyone who responded, on and off the list. I thought I'd post
> a quick summary.
>
> To clarify my question slightly (if belatedly) I'm specifi
Thanks to everyone who responded, on and off the list. I thought I'd post a
quick summary.
To clarify my question slightly (if belatedly) I'm specifically looking for a
passive 3D solution to plug into my MacBook Pro whilst on sabbatical in the
USA. I'll be using it for a mixture of 2D and 3D v
Dear Jacob Keller,
As you say, there is a number of methods used to assign, as correctly as
possible, ions and water molecules:
> depending of everything you know about the purification protocol of
the molecule (every ions and compound which could have been seen by the
target protein),
> depe
On Thu, Mar 6, 2014 at 11:45 AM, Keller, Jacob wrote:
> I was curious whether there has been a rigorous evaluation of ion binding
> sites in the structures in the pdb, by PDB-REDO or otherwise. I imagine
> that there is a considerably broad spectrum of habits and rigor in
> assigning solute blobs
Certainly some sites will be defined better than others by such
criteria. I would hope that they are discussed in the publications
relevant to the PDB submission.
On 03/06/14 14:45, Keller, Jacob wrote:
Dear Crystallographers,
I was curious whether there has been a rigorous evaluation of io
Dear Crystallographers,
I was curious whether there has been a rigorous evaluation of ion binding sites
in the structures in the pdb, by PDB-REDO or otherwise. I imagine that there is
a considerably broad spectrum of habits and rigor in assigning solute blobs to
ion X or water, and in fact it w
Every now and then I pipe on this as well. We have Zalman monitors here - I
bought them about 4 years ago - when you could not buy them any more in the
states. I just bought them on Ebay - they came new in the box directly from
China. The person did fed-ex shipping - did not cost any extra, a
Co expression of binding partner perhaps ? And then tagging the other protein
partner ?
Jürgen
..
Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
*WWTF-funded positions in the Life Sciences, hosted by the Max F. Perutz
Laboratories (MFPL), Vienna, Austria*
The Vienna Science and Technology Fund (*WWTF*) has launched a call for
applications in the Life Sciences.
The "Vienna Research Groups for Young Investigators" call by the City of
V
I beg to differ on this:
"Also, passive screens have a pol-filter in place, the fine lines of which you
will observe on a white background, the more disturbing the closer the viewing
distance to the screen is. So, for general office applications (writing text),
the screens are less useful. "
Our
Hi,
this is probaly due to the transition from old TFT style to new
IPS panel based monitors... Several new passive 3d monitors are
hitting the market such as AOC d2769Vh and the
Philips
Gioco 278G4. Both are based on 27" IPS Monitor
pa
Dear Wenhe,
this sounds a bit like our struggles to solubly express and purify the
dsRNA-binding avian reovirus sigmaA protein, please find the two papers here:
http://www.ncbi.nlm.nih.gov/pubmed/?term=van+Raaij+MJ%2C+sigmaA
The key to solving our problem was to realise the protein aggregated esp
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