Re: [ccp4bb] protocol for seleno-methionine incorporation...

2014-06-24 Thread Zhijie Li
Hi Mintu, I suggest using purified whole protein for ESI-MS. You will get a series of protein peaks (if the substitution is not 100%, which is often the case), each differ for 47 Dalton. You can determine how many Se each peak corresponds to if you know the molecular weight of your protein.

[ccp4bb] sharp error

2014-06-24 Thread Almudena Ponce Salvatierra
Hi everyone, I am running autosharp and in the first round of sharp, right after finding the heavy atoms sites, I get the following error: ***SHARP-ERR-0001: Error in bomb : STATUS = TEXT_CHANGED *** does anyone know what it means and how to fix it? Thanks a lot in advance. Best,

Re: [ccp4bb] sharp error

2014-06-24 Thread Gerard Bricogne
Dear Almudena, Thank you for your e-mail. It would be best to send this kind of message to sharp-deve...@globalphasing.com as this is very specific to SHARP. This looks like a mundane matter, to do with your .licence file. Could you send it to us so that we can take a look? Please send

[ccp4bb] Postdoctoral position in ligand-receptor interaction mechanisms in inflammation

2014-06-24 Thread Thomas Y. Cho
Laboratory of Thomas Yoonsang Cho in the Department of Biochemistry and Molecular Biology at Saint Louis University School of Medicine invites a highly motivated and creative postdoctoral fellow who is enthusiastic for the structural and functional characterization of ligand-receptor interaction