Hi Mintu,
I suggest using purified whole protein for ESI-MS. You will get a series of
protein peaks (if the substitution is not 100%, which is often the case), each
differ for 47 Dalton. You can determine how many Se each peak corresponds to if
you know the molecular weight of your protein.
Hi everyone,
I am running autosharp and in the first round of sharp, right after finding
the heavy atoms sites, I get the following error:
***SHARP-ERR-0001: Error in bomb : STATUS = TEXT_CHANGED ***
does anyone know what it means and how to fix it?
Thanks a lot in advance.
Best,
Dear Almudena,
Thank you for your e-mail. It would be best to send this kind of
message to sharp-deve...@globalphasing.com as this is very specific to SHARP.
This looks like a mundane matter, to do with your .licence file.
Could you send it to us so that we can take a look? Please send
Laboratory of Thomas Yoonsang Cho in the Department of Biochemistry and
Molecular Biology at Saint Louis University School of Medicine invites a
highly motivated and creative postdoctoral fellow who is enthusiastic for
the structural and functional characterization of ligand-receptor interaction