Re: [ccp4bb] protocol for seleno-methionine incorporation...
Hi Mintu, I suggest using purified whole protein for ESI-MS. You will get a series of protein peaks (if the substitution is not 100%, which is often the case), each differ for 47 Dalton. You can determine how many Se each peak corresponds to if you know the molecular weight of your protein. Then you can integrate the peaks to get an overall incorporation percentage. Zhijie From: Mintu Chandra Sent: Monday, June 23, 2014 11:57 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] protocol for seleno-methionine incorporation... Dear all, Is there any standard protocol to know the number of incorporated seleno-methionine in a protein ??? I am growing one of the protein in minimal media for phase determination. I am using mass spectrometry facility to know the incorporated seleno-methionine but I wanted to know whether I should use the purified protein or digested protein for mass spectrometry. Thanks, Mintu Chandra. -- Mintu Chandra Senior Research Fellow IISER BHOPAL mi...@iiserb.ac.in +918085288853
[ccp4bb] sharp error
Hi everyone, I am running autosharp and in the first round of sharp, right after finding the heavy atoms sites, I get the following error: ***SHARP-ERR-0001: Error in bomb : STATUS = TEXT_CHANGED *** does anyone know what it means and how to fix it? Thanks a lot in advance. Best, Almudena. -- Almudena Ponce-Salvatierra Macromolecular crystallography and Nucleic acid chemistry Max Planck Institute for Biophysical Chemistry Am Fassberg 11 37077 Göttingen Germany
Re: [ccp4bb] sharp error
Dear Almudena, Thank you for your e-mail. It would be best to send this kind of message to sharp-deve...@globalphasing.com as this is very specific to SHARP. This looks like a mundane matter, to do with your .licence file. Could you send it to us so that we can take a look? Please send it to the sharp-develop mailing list mentioned above, not to the ccp4bb list. With best wishes, Gerard. -- On Tue, Jun 24, 2014 at 05:46:42PM +0200, Almudena Ponce Salvatierra wrote: Hi everyone, I am running autosharp and in the first round of sharp, right after finding the heavy atoms sites, I get the following error: ***SHARP-ERR-0001: Error in bomb : STATUS = TEXT_CHANGED *** does anyone know what it means and how to fix it? Thanks a lot in advance. Best, Almudena. -- Almudena Ponce-Salvatierra Macromolecular crystallography and Nucleic acid chemistry Max Planck Institute for Biophysical Chemistry Am Fassberg 11 37077 Göttingen Germany
[ccp4bb] Postdoctoral position in ligand-receptor interaction mechanisms in inflammation
Laboratory of Thomas Yoonsang Cho in the Department of Biochemistry and Molecular Biology at Saint Louis University School of Medicine invites a highly motivated and creative postdoctoral fellow who is enthusiastic for the structural and functional characterization of ligand-receptor interaction mechanisms in inflammatory diseases. The successful candidate is expected to have strong X-ray crystallography background and will be engaged in the crystal structure determination of protein complexes and the cell biological elucidation of the downstream signaling pathways. As for the cell biological functional studies, we use dynamic mass redistribution (DMR) technology (Nat Biotechnol 2010 Schroder). Please visit our lab website for more details about our research ( http://biochem.slu.edu/faculty/cho/). Active projects are highly collaborative with medicinal chemists and immunologists for the development of novel agonists and antagonists for promising therapeutic targets. The laboratory is located in the state of the art Doisy Research Center (DRC) on the medical campus of Saint Louis University. The department has three R-Axis IV++ imaging plate systems for high-resolution X-ray diffraction data collection, crystallization robot, differential scanning calorimeter, isothermal titration calorimeter, dynamic lightscattering, metabolomics core facility, and bioinformatics facility ( http://biochemweb.slu.edu/index.shtml). In the same building, are animal care facility, flow cytometry research core, animal imaging core, microarray core, research microscopy and confocal core, and the Center for World Health and Medicine, which provides a full range of support for drug discovery programs including design and synthesis of small molecules and their preclinical characterization. Candidates must hold a Ph.D. in X-ray crystallography, biochemistry or a related field. Solid knowledge and hands-on experience in signaling studies with immune cells expressing cytokine/chemokine receptors is a plus. He or she must have excellent skills in oral and written English as well as inherent ability to organize experimental data. Applicants should send a cover letter with their brief scientific background and motivation for the position along with a complete CV, including research experience and the names and contact information of three references to Thomas Cho via email (yc...@slu.edu).
