Re: [ccp4bb] Pseudo-translation problem

Hi, Lattice-translocation disorder is one possibility. How does the diffraction image look like? Are there any streaks? Best regards, Takanori Nakane On 2014-07-30 15:58, Minyun Zhou wrote: Dear all, I am trying to determine the structure of a protein-DNA complex. I collected several

[ccp4bb] Post-doc position in Leeds

Dear All, A 3- year post-doctoral position is available immediately to work on the structural biology of ssRNA viruses and their assembly in my lab at the University of Leeds. We already have several structures determined by cryo-EM and single particle image processing, at resolutions ranging

[ccp4bb] Multiple pseudotranslations vectors in P1

Dear all, we are currently in the process of solving the structure of a Fab/peptide complex. We have first determined the structure of the native Fab and refined it to ~2A, so we have a decent MR model. The complex crystals that we are working on right now diffract to ~3.2A and are

[ccp4bb] MR: space group and cell units

Dear All: I try to solve a structure by using Phaser. The data set was collected at 3.9 A with 98.1% (95%) completeness. Based on pointless, the best space group for the data set is P21212. And the data can be processed with P21, P212121 as well. The I/sigma value for the data is 13.3 (1.9).

[ccp4bb] Protein-DNA structure solution

Hello CCP4bb I am solving a 3.2A resolution crystal data for a protein DNA complex. I would like to know- 1) How to validate the DNA constraints in the crystal structure? 2) How to fit DNA into density? How different it is from protein fitting into density? 3) What kind of parameters to consider

Re: [ccp4bb] Multiple pseudotranslations vectors in P1

Dear Thomas, At the moment, Phaser only deals fully automatically with 2-fold tNCS (i.e. one unique peak in the native Patterson), although we’re hoping to make it more general. With some tailored input, it can also deal with the case of several copies related by multiples of the same NCS

Re: [ccp4bb] MR: space group and cell units

HI, Mark: Thank you for your suggestions. try all possible spacegroups with PHASER Yes, this is done with all the modeling. make sure you are using a monomer as search model I also tried with monomer as search model. When run with refmac5, the R-value is above 0.5. I exam the model in coot.

[ccp4bb] Postdoctoral position available in the area of Membrane Transport at Northwestern University

Postdoctoral position available in the area of Membrane Transport at Northwestern University The Pinkett laboratory at Northwestern University is seeking a highly motivated candidate for a postdoctoral fellow position in the field of protein structural biology. The individual will join a