[ccp4bb] Protein precipitating at higher concentration!!
Hi Xtallographers, I have been able to purify a protein that was initially going into inclusion bodies from the excellent suggestions that I got here. So my lysis buffer has 0.5M Guanidium Hydrochoride, 2% TritonX-100, 500mM NaCl, 5% Glycerol in 20 mM Tris-HCL pH 8.0 The problem is that the protein is first purified as a SUMO-fusion protein which is further proteolysed and passed through the Talon resin to get the final SUMO-Free construct. However as I have around 250mM Imidazole (pH elution did not work) from the elution of the first round, I have to dialyse the sample to get rid of the imidazole so that I can use the proteolysed sample again on the column. All these I do in a buffer that does not have GuHCL or Triton. However I have kept the NaCl concentration same (0.5 M). I start to see white insoluble precipitate right from the dialysis step. If I spin the precipitate out, I still have a lot of protein to go to the next step of proteolysis. The problem is that when I finally want to concentrate the protein to run the SEC step, all my protein starts precipitating starting from 5mg/ml to all the way to 25-30 mg/ml. Are there some smart ways to keep the protein soluble at higher concentrations, assuming that I do not have to remove them before setting up trays? Should I keep on using Guanidium Hcl and Triton for all the steps all the way into the SEC column. Have people got any good results using 5% Acetronitrile, 50mM Arginine or DTT? (used for NMR samples) Any help in this regard will be very helpful. The protein is an engineered bacterial transcription factor. (not a membrane protein) Thanks in advance as always, ivan
Re: [ccp4bb] active 3D monitors: successor of Asus VG278HR?
In addition to what others have -- correctly -- stated I want to add one more thing: Yes, you are right, if you do not get your hands on a monitor with built-in emitter, you'll need ad least a K4000 and in many cases the VESA din bracket (~$50). You do not have to buy the expensive ($800+) 3D Vision pro emitter, though, for about $150 you can get the 3D Vision 2 (the 2 is important!) kit, that includes the DIN-to-Phone jack cable (officially for connection to DLP) you'll need to connect the graphics card to the emitter. Don't use the DP-DVI adapter, there's not enough bandwidth - go straight out of the DVI and you'll be fine (this realization cost me a day). HTH, Jens On Thu, 2015-01-08 at 15:08 +0100, Tobias Beck wrote: Dear all, I am looking again at 3D monitors. Last year I bought for my old lab the VG278HR and the PNY K600, as advised by the CCP4BB. (The 3D test images from Nvidia were running fine under Windows, but I did not get around to finish the set up with pymol and coot under linux.) Now at a new place, I looked at available monitors again (that have the built-in emitter since I want to use the K600 graphics card) and noticed that the VG278HR is out of stock. The VG278H, which seems to be a very similar model, is also out of stock. This page http://www.nvidia.com/object/3d-vision-displays.html also lists the Acer HN274H as a 27'' monitor with built-in emitter, but that seems to be out of stock as well (I would prefer not to buy a refurbished or used one). The monitors mentioned above are also listed here: http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Stereo (The smaller BenQ XL2420TX listed there is also out of stock). Has anybody ordered a 3D monitor with built-in emitter recently or could provide me with a current list of 27'' monitors with built-in emitters? I checked with Nvidia via their chat support, but they did not have an updated list, just provided links to the manufacturers' homepages. If monitors with built-in emitters are not available anymore, I need to buy a different graphics card in order for the setup to work with linux, right? Thank you and best wishes, Tobias. -- ___ Dr. Tobias Beck - group leader - RWTH Aachen University Institute of Inorganic Chemistry Landoltweg 1, office: 304N 52056 Aachen, Germany phone: +49-241-80-90057 fax: +49-241-80-99003 ___
[ccp4bb] Bulk solvent
Dear all, Is there any reason for using Babinet scaling for bulk solvent correction instead of mask based scaling? Armando
Re: [ccp4bb] Protein precipitating at higher concentration!!
Hi Ivan this will not be an answer to your question, but did you consider not concentrating 'too much' your sample? It happened few times to me that the protein was precipitating when concentrating for SEC because of the presence of other impurities. Trying the good old AS precipitation helped a bit, but wasn't really the magical solution as I was losing a bit of the protein of interest as well. The solution was to concentrate only slightly the sample, and pass it though multiple (at the time it was quite a lot actually) runs of SEC. I ended up with again a lot of pure sample to concentrate, however, this sample was pure enough and did not precipitate. Other than that, I guess playing with the salt concentration might help keeping things stable... or not. I know people also tried the addition of glycerol or EG, but I don't have personal experience in that and cannot really comment if it is working well or not. Cheers, Leo On Jan 9, 2015, at 9:00 AM, xaravich ivan xaravich.i...@gmail.com wrote: Hi Xtallographers, I have been able to purify a protein that was initially going into inclusion bodies from the excellent suggestions that I got here. So my lysis buffer has 0.5M Guanidium Hydrochoride, 2% TritonX-100, 500mM NaCl, 5% Glycerol in 20 mM Tris-HCL pH 8.0 The problem is that the protein is first purified as a SUMO-fusion protein which is further proteolysed and passed through the Talon resin to get the final SUMO-Free construct. However as I have around 250mM Imidazole (pH elution did not work) from the elution of the first round, I have to dialyse the sample to get rid of the imidazole so that I can use the proteolysed sample again on the column. All these I do in a buffer that does not have GuHCL or Triton. However I have kept the NaCl concentration same (0.5 M). I start to see white insoluble precipitate right from the dialysis step. If I spin the precipitate out, I still have a lot of protein to go to the next step of proteolysis. The problem is that when I finally want to concentrate the protein to run the SEC step, all my protein starts precipitating starting from 5mg/ml to all the way to 25-30 mg/ml. Are there some smart ways to keep the protein soluble at higher concentrations, assuming that I do not have to remove them before setting up trays? Should I keep on using Guanidium Hcl and Triton for all the steps all the way into the SEC column. Have people got any good results using 5% Acetronitrile, 50mM Arginine or DTT? (used for NMR samples) Any help in this regard will be very helpful. The protein is an engineered bacterial transcription factor. (not a membrane protein) Thanks in advance as always, ivan
Re: [ccp4bb] CCP4 Study Weekend 2015 Advances in Experimental Phasing - Live Web Streaming
Hi, Harry, (Happy New Year) In fact, yes, the ‘what’s new’ sessions are being webcast: I see it fine and dandy. Hopefully, the problem encountered / reported by Tereza is overcome by now. Very Best Regards, Marcus. From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Harry Powell Sent: Thursday, January 08, 2015 10:34 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] CCP4 Study Weekend 2015 Advances in Experimental Phasing - Live Web Streaming I'm not sure the what's new sessions are webcast - try again when the main sessions are due to start at 11 am local time (in about 25 minutes time). Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge, CB2 0QH Chairman of European Crystallographic Association SIG9 (Crystallographic Computing) On 8 Jan 2015, at 09:37, Tereza Skalova t.skalova.c...@gmail.commailto:t.skalova.c...@gmail.com wrote: Do you see the video? I see nothing. Tereza Skalova 2015-01-07 15:32 GMT+01:00 Ronan Keegan ronan.kee...@stfc.ac.ukmailto:ronan.kee...@stfc.ac.uk: Dear All, The webcast for this year's CCP4 Study Weekend on Advances in Experimental Phasing taking place on the 8/9 of January will be available from the STFC Webinars page: http://www.stfc.ac.uk/webinars A programme for the meeting can be found here: http://www.ccp4.ac.uk/events/CCP4_2015/programme.html Best wishes, Ronan
Re: [ccp4bb] CCP4 Study Weekend 2015 Advances in Experimental Phasing - Live Web Streaming
Now it works. Thanks for all advices. Tereza Skalova 2015-01-08 10:37 GMT+01:00 Tereza Skalova t.skalova.c...@gmail.com: Do you see the video? I see nothing. Tereza Skalova 2015-01-07 15:32 GMT+01:00 Ronan Keegan ronan.kee...@stfc.ac.uk: Dear All, The webcast for this year's CCP4 Study Weekend on Advances in Experimental Phasing taking place on the 8/9 of January will be available from the STFC Webinars page: http://www.stfc.ac.uk/webinars A programme for the meeting can be found here: http://www.ccp4.ac.uk/events/CCP4_2015/programme.html Best wishes, Ronan
Re: [ccp4bb] CCP4 Study Weekend 2015 Advances in Experimental Phasing - Live Web Streaming
Hi, I watched the live stream of what's new sessions without any problem from my university's network. The video uses RTMP streaming, not HTTP. Some network might have difficulty accessing it. The recordings will be provided in RTMP and HTTP, if they don't change settings from 2014. Best regards, Takanori Nakane On 2015/01/08 19:34, Harry Powell wrote: I'm not sure the what's new sessions are webcast - try again when the main sessions are due to start at 11 am local time (in about 25 minutes time). Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge, CB2 0QH Chairman of European Crystallographic Association SIG9 (Crystallographic Computing) On 8 Jan 2015, at 09:37, Tereza Skalova t.skalova.c...@gmail.com wrote: Do you see the video? I see nothing. Tereza Skalova 2015-01-07 15:32 GMT+01:00 Ronan Keegan ronan.kee...@stfc.ac.uk: Dear All, The webcast for this year's CCP4 Study Weekend on Advances in Experimental Phasing taking place on the 8/9 of January will be available from the STFC Webinars page: http://www.stfc.ac.uk/webinars A programme for the meeting can be found here: http://www.ccp4.ac.uk/events/CCP4_2015/programme.html Best wishes, Ronan
Re: [ccp4bb] CCP4 Study Weekend 2015 Advances in Experimental Phasing - Live Web Streaming
Do you see the video? I see nothing. Tereza Skalova 2015-01-07 15:32 GMT+01:00 Ronan Keegan ronan.kee...@stfc.ac.uk: Dear All, The webcast for this year's CCP4 Study Weekend on Advances in Experimental Phasing taking place on the 8/9 of January will be available from the STFC Webinars page: http://www.stfc.ac.uk/webinars A programme for the meeting can be found here: http://www.ccp4.ac.uk/events/CCP4_2015/programme.html Best wishes, Ronan
Re: [ccp4bb] CCP4 Study Weekend 2015 Advances in Experimental Phasing - Live Web Streaming
I'm not sure the what's new sessions are webcast - try again when the main sessions are due to start at 11 am local time (in about 25 minutes time). Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge, CB2 0QH Chairman of European Crystallographic Association SIG9 (Crystallographic Computing) On 8 Jan 2015, at 09:37, Tereza Skalova t.skalova.c...@gmail.com wrote: Do you see the video? I see nothing. Tereza Skalova 2015-01-07 15:32 GMT+01:00 Ronan Keegan ronan.kee...@stfc.ac.uk: Dear All, The webcast for this year's CCP4 Study Weekend on Advances in Experimental Phasing taking place on the 8/9 of January will be available from the STFC Webinars page: http://www.stfc.ac.uk/webinars A programme for the meeting can be found here: http://www.ccp4.ac.uk/events/CCP4_2015/programme.html Best wishes, Ronan
Re: [ccp4bb] CCP4 Study Weekend 2015 Advances in Experimental Phasing - Live Web Streaming
Yes, this may be the reason. I solved my problem to see the video by changing house. It did not work on any computer at work (Windows7, Linux). It works at home (Windows 8.1 and different network). Tereza Skalova 2015-01-08 12:39 GMT+01:00 Takanori Nakane takanori.nak...@bs.s.u-tokyo.ac.jp: Hi, I watched the live stream of what's new sessions without any problem from my university's network. The video uses RTMP streaming, not HTTP. Some network might have difficulty accessing it. The recordings will be provided in RTMP and HTTP, if they don't change settings from 2014. Best regards, Takanori Nakane On 2015/01/08 19:34, Harry Powell wrote: I'm not sure the what's new sessions are webcast - try again when the main sessions are due to start at 11 am local time (in about 25 minutes time). Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge, CB2 0QH Chairman of European Crystallographic Association SIG9 (Crystallographic Computing) On 8 Jan 2015, at 09:37, Tereza Skalova t.skalova.c...@gmail.com wrote: Do you see the video? I see nothing. Tereza Skalova 2015-01-07 15:32 GMT+01:00 Ronan Keegan ronan.kee...@stfc.ac.uk: Dear All, The webcast for this year's CCP4 Study Weekend on Advances in Experimental Phasing taking place on the 8/9 of January will be available from the STFC Webinars page: http://www.stfc.ac.uk/webinars A programme for the meeting can be found here: http://www.ccp4.ac.uk/events/CCP4_2015/programme.html Best wishes, Ronan
Re: [ccp4bb] active 3D monitors: successor of Asus VG278HR?
I'll second Jim on this. Just this week, I set up my linux box with the nvidia 3d vision 2 kit which I use with the ASUS VG278HE. My graphics card is the quadro K4000 and the only pitfall was that I had to purchase the 3 pin connector to get it to sync: http://www.cdw.com/shop/products/PNY-full-height-bracket/2277933.aspx Good Luck- Todd From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Jim Fairman [fairman@gmail.com] Sent: Thursday, January 08, 2015 9:52 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] active 3D monitors: successor of Asus VG278HR? Hi Tobias, You most definitely do not need a built-in emitter for your system to work. Instead you use the little emitter temple that comes with a set of the glasseshttp://www.nvidia.com/object/product-geforce-3d-vision2-wireless-glasses-kit-us.html. In Windows, the emitter can be driven via USB, but for Linux it will need to be driven by the 3-pin stereo connector that plugs directly into your Quadro-class graphics card. You can just place the emitter anywhere that the glasses have a clear line of sight to while you are wearing them. I usually pick directly underneath the screen. The ASUS VG278HEhttp://www.amazon.com/VG278HE-27-Inch-Screen-LED-lit-Monitor/dp/B00906HM6K/ref=sr_1_1?ie=UTF8qid=1420732033sr=8-1keywords=ASUS+VG278HE (without built in emitter) is in stock over at Amazon. Cheers, Jim On Thu, Jan 8, 2015 at 6:08 AM, Tobias Beck tobiasb...@gmail.commailto:tobiasb...@gmail.com wrote: Dear all, I am looking again at 3D monitors. Last year I bought for my old lab the VG278HR and the PNY K600, as advised by the CCP4BB. (The 3D test images from Nvidia were running fine under Windows, but I did not get around to finish the set up with pymol and coot under linux.) Now at a new place, I looked at available monitors again (that have the built-in emitter since I want to use the K600 graphics card) and noticed that the VG278HR is out of stock. The VG278H, which seems to be a very similar model, is also out of stock. This page http://www.nvidia.com/object/3d-vision-displays.html also lists the Acer HN274H as a 27'' monitor with built-in emitter, but that seems to be out of stock as well (I would prefer not to buy a refurbished or used one). The monitors mentioned above are also listed here: http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Stereo (The smaller BenQ XL2420TX listed there is also out of stock). Has anybody ordered a 3D monitor with built-in emitter recently or could provide me with a current list of 27'' monitors with built-in emitters? I checked with Nvidia via their chat support, but they did not have an updated list, just provided links to the manufacturers' homepages. If monitors with built-in emitters are not available anymore, I need to buy a different graphics card in order for the setup to work with linux, right? Thank you and best wishes, Tobias. -- ___ Dr. Tobias Beck - group leader - RWTH Aachen University Institute of Inorganic Chemistry Landoltweg 1, office: 304N 52056 Aachen, Germany phone: +49-241-80-90057tel:%2B49-241-80-90057 fax: +49-241-80-99003tel:%2B49-241-80-99003 ___ -- Jim Fairman, Ph D. Group Leader I - Crystallography Berylliumhttp://www.be4.com Tel: 206-780-8914 Cell: 240-479-6575 E-mail: fairman@gmail.commailto:fairman@gmail.com jfair...@embios.commailto:jfair...@embios.com
Re: [ccp4bb] How far does rad dam travel?
Yes, this is great info and thoughts. What I still do not understand, however, is why the noise from air/loop scattering is so bad--why not make sure only the top of the Gaussian is engulfing the crystal, and the tails can hit air or loop? Isn't the air scattering noise easily subtractable, being essentially flat over time, whereas uneven illumination of the crystal is highly difficult to correct? Also, in light of these considerations, it would seem to me a much better use of resources not to make brighter and smaller beams but instead concentrate on making better low-intensity big beam profiles (top-hats?) and lower-noise, faster detectors (like Pilatus and the new ADSC). Jacob -Original Message- From: James Holton [mailto:jmhol...@lbl.gov] Sent: Tuesday, December 30, 2014 3:57 PM To: Keller, Jacob; CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] How far does rad dam travel? Yes, bigger is okay, and perhaps a little better if you consider the effects of beam/crystal vibration and two sharp-edged boundaries dancing over each other. But bigger is better only to a point. That point is when the illuminated area of non-good-stuff is about equal to the area of the good stuff. This is because the total background noise is equal to the square root of the number of photons and equal volumes of any given stuff (good or non-good) yield about the same number of background-scattered photons. So, since you're taking the square root, completely eliminating the non-good-stuff only buys you a gain of 40% in total noise. Given that other sources of noise come into play when the beam and crystal are exactly matched (flicker), 40% is a reasonable compromise. This is why I recommend loop sizes that are about 40% bigger than the crystal itself. Much less risk of surface-tension injury, and the air around the loop scatters 1000x less than the non-crystal stuff in the loop: effectively defining the beam size. As for what beam profiles look like at different beamlines, there are some sobering mug-shots in this paper: http://dx.doi.org/10.1107/S0909049511008235 Some interesting quirks in a few of them, but in general optimally focused beams are Gaussian. Almost by definition! (central limit theorem and all that). It is when you de-focus that things get really embarrassing. X-ray mirrors all have a fingerprint in the de-focused region that leads to striations and other distortions. The technology is improving, but good solutions for de focusing are still not widely available. Perhaps because they are hard to fund. Genuine top-hat beams are rare, but there are a few of them. Petra-III is particularly proud of theirs. But top-hats are usually defined by collimation of a Gaussian and the more x-rays you have hitting the back of the aperture the more difficult it is to control the background generated by the collimator. If you can see the shadow of your pin on the detector, then you know there is a significant amount of background that is coming from upstream of your crystal! My solution is to collimate at roughly the FWHM. This chops off the tails and gives you a tolerably flat beam in the middle. How much more intense is the peak than the tails? Well, at the FWHM, the intensity is, well, half of that at the center. At twice that distance from the center, you are down to 6.2%. The equation is exp(-log(16)*(x/hwhm)**2) where hwhm is 1/2 of the FHWM. HTH! -James Holton MAD Scientist On 12/30/2014 12:10 PM, Keller, Jacob wrote: Yes, it gets complicated, doesn't it? This is why I generally recommend trying to use a beam that matches your crystal size. ...or is bigger, right? Diffuse scattering, yes, but more even illumination might be worth it? Generally, James, I have a question: what is the nature of the intensity cross-sections at most beamlines--are they usually Gaussian, or are some flatter? Or I guess, if Gaussian, how much more intense is the peak than the tails? JPK
[ccp4bb] Demonstration for 2nd graders?
Hi everyone, Slightly off topic here but I got myself volunteered by my 2nd grade son to do a show and tell at his class. I have the rock candy experiment ready with some background info on what I do. Can anyone direct me to some resources or your personal demo's that you have done? Thanks a bunch -John
[ccp4bb] active 3D monitors: successor of Asus VG278HR?
Dear all, I am looking again at 3D monitors. Last year I bought for my old lab the VG278HR and the PNY K600, as advised by the CCP4BB. (The 3D test images from Nvidia were running fine under Windows, but I did not get around to finish the set up with pymol and coot under linux.) Now at a new place, I looked at available monitors again (that have the built-in emitter since I want to use the K600 graphics card) and noticed that the VG278HR is out of stock. The VG278H, which seems to be a very similar model, is also out of stock. This page http://www.nvidia.com/object/3d-vision-displays.html also lists the Acer HN274H as a 27'' monitor with built-in emitter, but that seems to be out of stock as well (I would prefer not to buy a refurbished or used one). The monitors mentioned above are also listed here: http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Stereo (The smaller BenQ XL2420TX listed there is also out of stock). Has anybody ordered a 3D monitor with built-in emitter recently or could provide me with a current list of 27'' monitors with built-in emitters? I checked with Nvidia via their chat support, but they did not have an updated list, just provided links to the manufacturers' homepages. If monitors with built-in emitters are not available anymore, I need to buy a different graphics card in order for the setup to work with linux, right? Thank you and best wishes, Tobias. -- ___ Dr. Tobias Beck - group leader - RWTH Aachen University Institute of Inorganic Chemistry Landoltweg 1, office: 304N 52056 Aachen, Germany phone: +49-241-80-90057 fax: +49-241-80-99003 ___
Re: [ccp4bb] active 3D monitors: successor of Asus VG278HR?
Hi Tobias, You most definitely do not need a built-in emitter for your system to work. Instead you use the little emitter temple that comes with a set of the glasses http://www.nvidia.com/object/product-geforce-3d-vision2-wireless-glasses-kit-us.html. In Windows, the emitter can be driven via USB, but for Linux it will need to be driven by the 3-pin stereo connector that plugs directly into your Quadro-class graphics card. You can just place the emitter anywhere that the glasses have a clear line of sight to while you are wearing them. I usually pick directly underneath the screen. The ASUS VG278HE http://www.amazon.com/VG278HE-27-Inch-Screen-LED-lit-Monitor/dp/B00906HM6K/ref=sr_1_1?ie=UTF8qid=1420732033sr=8-1keywords=ASUS+VG278HE (without built in emitter) is in stock over at Amazon. Cheers, Jim On Thu, Jan 8, 2015 at 6:08 AM, Tobias Beck tobiasb...@gmail.com wrote: Dear all, I am looking again at 3D monitors. Last year I bought for my old lab the VG278HR and the PNY K600, as advised by the CCP4BB. (The 3D test images from Nvidia were running fine under Windows, but I did not get around to finish the set up with pymol and coot under linux.) Now at a new place, I looked at available monitors again (that have the built-in emitter since I want to use the K600 graphics card) and noticed that the VG278HR is out of stock. The VG278H, which seems to be a very similar model, is also out of stock. This page http://www.nvidia.com/object/3d-vision-displays.html also lists the Acer HN274H as a 27'' monitor with built-in emitter, but that seems to be out of stock as well (I would prefer not to buy a refurbished or used one). The monitors mentioned above are also listed here: http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Stereo (The smaller BenQ XL2420TX listed there is also out of stock). Has anybody ordered a 3D monitor with built-in emitter recently or could provide me with a current list of 27'' monitors with built-in emitters? I checked with Nvidia via their chat support, but they did not have an updated list, just provided links to the manufacturers' homepages. If monitors with built-in emitters are not available anymore, I need to buy a different graphics card in order for the setup to work with linux, right? Thank you and best wishes, Tobias. -- ___ Dr. Tobias Beck - group leader - RWTH Aachen University Institute of Inorganic Chemistry Landoltweg 1, office: 304N 52056 Aachen, Germany phone: +49-241-80-90057 fax: +49-241-80-99003 ___ -- Jim Fairman, Ph D. Group Leader I - Crystallography Beryllium http://www.be4.com Tel: 206-780-8914 Cell: 240-479-6575 E-mail: fairman@gmail.com jfair...@embios.com
Re: [ccp4bb] Demonstration for 2nd graders?
This one is probably above second grade, but the equipment setup is pretty easy http://ipl.physics.harvard.edu/wp-uploads/2013/03/15c_s07_5.pdf Measuring the wavelength of light with a ruler On 01/08/15 13:35, John Lee wrote: Hi everyone, Slightly off topic here but I got myself volunteered by my 2nd grade son to do a show and tell at his class. I have the rock candy experiment ready with some background info on what I do. Can anyone direct me to some resources or your personal demo's that you have done? Thanks a bunch -John -- === All Things Serve the Beam === David J. Schuller modern man in a post-modern world MacCHESS, Cornell University schul...@cornell.edu
Re: [ccp4bb] Demonstration for 2nd graders?
When my daughter was in Kindergarten, her class took a trip to our facility, and I showed them some of my crystal trays (What do you see here? Do you see anything? Clear drops ..., they effectively said). Then I showed them through the microscope several crystals, and I was pleasantly surprised by their awe (oooh! Jewels!). Then I showed them some loops by-eye and by-microscope. I'd have liked to show them how I manipulate a crystal with that loop, but I wasn't set up to project it for everyone to see real-time. I then told them about protein machines that line up in a particular way to form that jewel they'd just seen. ...I imagine you cannot bring a good enough microscope into the classroom with you without some hassle, but I thought I'd share at least that 5-6 year olds, even, can find protein crystals very fascinating. Emily. On Thu, Jan 8, 2015 at 2:02 PM, David Schuller schul...@cornell.edu wrote: This one is probably above second grade, but the equipment setup is pretty easy http://ipl.physics.harvard.edu/wp-uploads/2013/03/15c_s07_5.pdf Measuring the wavelength of light with a ruler On 01/08/15 13:35, John Lee wrote: Hi everyone, Slightly off topic here but I got myself volunteered by my 2nd grade son to do a show and tell at his class. I have the rock candy experiment ready with some background info on what I do. Can anyone direct me to some resources or your personal demo's that you have done? Thanks a bunch -John -- === All Things Serve the Beam === David J. Schuller modern man in a post-modern world MacCHESS, Cornell University schul...@cornell.edu
Re: [ccp4bb] Demonstration for 2nd graders?
I like the sodium acetate crystallization from Dr. Shakhashiri’s Chemical Demonstrations. I prepare the supersaturated solution ahead of time, then seed it with crystals during class. It’s fast, simple, entertaining, and the flask gets warm, showing that heat is being released by the reaction. Here’s the link: http://lecturedemos.chem.umass.edu/solutions13_3.html. From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of David Schuller Sent: Thursday, January 08, 2015 1:02 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Demonstration for 2nd graders? This one is probably above second grade, but the equipment setup is pretty easy http://ipl.physics.harvard.edu/wp-uploads/2013/03/15c_s07_5.pdf Measuring the wavelength of light with a ruler On 01/08/15 13:35, John Lee wrote: Hi everyone, Slightly off topic here but I got myself volunteered by my 2nd grade son to do a show and tell at his class. I have the rock candy experiment ready with some background info on what I do. Can anyone direct me to some resources or your personal demo's that you have done? Thanks a bunch -John -- === All Things Serve the Beam === David J. Schuller modern man in a post-modern world MacCHESS, Cornell University schul...@cornell.edumailto:schul...@cornell.edu
Re: [ccp4bb] Demonstration for 2nd graders?
I have had young ones grow lysozyme crystals in just a few minutes, using eye droppers and petri dishes. The crystals grow very fast, you can watch them grow in the microscope. Also they grow large enough you can see them by eye. Some izit dye would be fun to add (never did that). Then I let them take the setups home with them (nothing toxic in it). They all wanted to take them home. Stock solutions: 100 mg/ml sigma lysozyme in 50 mM sodium Acetate pH 4.5 4 M stock NaCl 50% w/v MPEG 5,000 1 M stock sodium acetate pH 4.5 Reservoir MasterMix for 60 reactions 9 mL water 22.5 mL NaCl 4.5 mL sodium acetate 54 mL MPEG with transfer pipette into small 35X10 mm Petri dish 1 drop protein + 1 drop reservoir Mix equal amount of lysozyme with reagent. Dilute protein and/or MPEG and/or Sodium Chloride for less nucleation, larger, and better shaped crystals. On Thu, Jan 8, 2015 at 1:02 PM, David Schuller schul...@cornell.edu wrote: This one is probably above second grade, but the equipment setup is pretty easy http://ipl.physics.harvard.edu/wp-uploads/2013/03/15c_s07_5.pdf Measuring the wavelength of light with a ruler On 01/08/15 13:35, John Lee wrote: Hi everyone, Slightly off topic here but I got myself volunteered by my 2nd grade son to do a show and tell at his class. I have the rock candy experiment ready with some background info on what I do. Can anyone direct me to some resources or your personal demo's that you have done? Thanks a bunch -John -- === All Things Serve the Beam === David J. Schuller modern man in a post-modern world MacCHESS, Cornell University schul...@cornell.edu
