Postdoctoral Research Fellow in Structural Biology of Hsp90 complexes (Fixed
term) Ref 143
http://www.sussex.ac.uk/aboutus/jobs/143
School of Life Sciences - Genome Damage and Stability Centre
Fixed term for 3 years, full time.
Salary range: starting at £31,342 and rising to £37,394 per
Dear All,
Sorry for the off topic question but I would like to hear from this group
experiences they have with sample cooling systems for x-ray data collection.
We have had a x-stream from Rigaku since ~1999 and have been very happy with
it. Unfortunately it is now obsolete since we can not
The mercury lamp (28-4042-25) offered by GE Healthcare for Akta systems come
with the housing and is priced at $1345. We have plenty of sparing housing
units and are interested in just the lamp. Does anyone here have any cheaper
alternatives/suggestions than buying the lamp and housing?
Thanks
I wouldn't mind knowing how to source this lamp as well. But FYI, the
lamps are usable for years after the FPLC gives you the dire low
intensity warning. We just ignore it until the lamp goes completely
dark or it's impossible to measure normal absorbances. We've used the
current lamp for
I am not sure if you have anyone electronically-minded at your disposal,
however there are now LEDs available at 260 and 280nm peak wavelengths.
They cost ~$40-$120 each (my data are at least 6 months old so I am not
sure if they still cost the same) and they should, with a little fiddling,
be
Along with all the excellent suggestions so far you can also try no
cryoprotectant at all: If you harvest your crystal on a mesh loop and
remove all the mother liquor the crystal lattice itself will act as a
cryoprotectant as long as the solvent channels are smaller than 40A in
diameter (they
You could try adding TEV to your protein just prior to crystallisation. I
produced 2 equivalent structures this way; one with the tag clearly visible
packing between molecules in an I centred space group and a 2nd (with TEV
added) in P21 where the tag was no longer visible (the packing
I’ll add to this that you should also set the relevant path in launchd.conf as
well to access other external programs (SHELXC/D/E) when opening ccp4i via the
Finder/Spotlight.
setenv PATH /where/ever/shelx
Cheers,
Aaron
--
Dr. Aaron Finke
Postdoctoral
Cryo
On 5 May 2015 13:51, faisaltari...@gmail.com wrote:
Thank you everyone..I have got some hints from this discussion and will
definitely try some of them to check its efficacy for my case...Thanx again
for your valuable suggestions..
On 5 May 2015 13:48, Anthony Savill
Dear Mark,
Indeed there is a way. You have to set the environment variable so that all Mac
apps can access it. This is most easily done via launchd.
In the Terminal, type ‘sudo vi /etc/launchd.conf’
and add the following text (the file will likely be blank):
setenv SHARP_home
Hi,
I would like to come into contact with users of the Formulatrix NT8, especially
if they have the LCP option. If you have one, please contact me off-list.
Best regards
Derek
Derek Logan
Hi Faisal,
Did you try to simply raise the Sokalan CP7 percentage? Additives like
Glycerol can increase the solubility of proteins, in that case you
have to counteract by increasing also the precipitant concentration.
If your crystals crack, a likely reason is osmotic shock. Particularly
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