Hi Ben
From discussions we have had with PDBe they consider tautomers to be
different compounds (just as stereoisomers would be considered to be
different compounds), since they require different restraint dictionaries,
so each tautomer that was observed would require a unique 3-lettter code.
Of
Hi folkd,
We have just collected some data and was busy processing and ran aimless having
updated to the latest versions (upgrades 10 and 11 I think). Both aimless and
then scala stops/fails almost immediately without writing any log files etc. I
tried running the same unmerged mtz on an older
Another major problem with the PDB is that it does not seem to believe in the
existence of different tautomers or protonation states.
For example the ATP analogue AMPPNP can have the nitrogen between the beta and
gamma phosphates protonated (-P-NH-P) or unprotonated (P-N=P), and there are
well
It's hard to know without more information. If there really is no log file,
then that suggests the job hasn't even started, so maybe something wrong with
the ccp4i interface
Which task were you using?
1. Symmetry, Scale, Merge (Aimless), the recommended one, or
2. Find Symmetry, Scale and
Please note the opening for post-doctor positions at UCMR, Umeå University.
Best wishes
Elisabeth Sauer-Eriksson
-
Postdoctoral Research Opportunities in Infection Biology and Molecular
Infection Medicine at Umeå University, Sweden
Deadline:
Good afternoon both,
there is also the issue of inconsistency of presentation.
For example, Lysine, that is L-Lysine (LYS) is protonated on the side
chain nitrogen (NZ), whiles as D-lysine (DLY) is not.
i.e. you have NZ(HZ1, HZ2) for DLY, and NZ(HZ1, HZ2, HZ3) for LYS
Miri
On Wed, 2015-06-24
Hi Sreetama,
It's very hard for anyone to help you out without more specific
information. A quick google gets me a couple of potentially relevant hits:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3595323/
http://www.ncbi.nlm.nih.gov/pubmed/8650159
http://www.ncbi.nlm.nih.gov/pubmed/10218107
Hello BBians,
sorry for the off-topic question. I have plasmid which possess the
precision protease site to get rid of GST tag. Does anybody have precision
protease plasmid. If someone has please share with me. I will be very
thankful for you.
Neeraj
--
=
Neeraj Kr.
