Hello, everyone，o:p/o:pnbsp;Recently, we found an intermolecular disulfide
bond from different symmetric units. I want to define it to refine, such as by
refmac5. nbsp;I know the regular format of a disulfide bond in the same
symmetric unit and show it as below.o:p/o:pnbsp;SSBONDnbsp;nbsp; 1
Dear Stefan,
just saw this after reading post:
http://www.nature.com/nature/journal/v522/n7557/full/nature14559.html
Best, Guenter
Pramod,
You already got good suggestions on how to handle DNA contamination in protein
preparations.
Let me point out briefly that you haven't demonstrated yet
Can anyone suggest a method/program for analysing conserved waters between two
related protein structures? Rex Palmer
http://www.bbk.ac.uk/biology/our-staff/emeritus-staff
http://www.springer.com/978-1-4614-3954-7
you add something like 2555 to define the required symmetry operator on
the ssbond line. - the exact format is described in the pdb convention.
But I think refmac does it i think if you as it to make disulphides.
Eleanor
On 28 June 2015 at 09:33, Zhongzhou Chen zhongzhouc...@sohu.com wrote:
Dear Prof. Eleanornbsp;Dodson,o:p/o:p
o:pnbsp;/o:p
nbsp;Thanks for your good suggestion.o:p/o:p
It works for my
pdb.o:p/o:p
o:pnbsp;/o:p
Best,o:p/o:pzhongzhougt;nbsp;you add something like 2555 to define the
required symmetry operator on the ssbond line. - the exact format is described
in
Thanks Jurgen,
But there is no space in the last directory, I do not know where it is
coming from.
Its really making me crazy, because I have 0.95 Angs data of a long
peptide, I wanted to solve it over the weekend!!
I have no clue why this is happening
Thanks for your suggestion!
On Sun, Jun 28,
Thanks for your suggestion, Jurgen
I tried xia2 and tried it several times, turning the machine off,
rebooting, using different data sets, exiting ccp4i but every time it gives
this error msg.(attached)
On Sun, Jun 28, 2015 at 2:16 PM, Jurgen Bosch jbos...@jhu.edu wrote:
Try xia2 from ccp4i
